超微粉碎辅助制备菜籽饼活性肽工艺优化及其抗氧化活性分析  

Optimization and Antioxidant Activity of Peptides from Rapeseed Cake Assisted by Superfine Grinding

作　　者：翟晓娜 胡龙彪 李媛媛 周新群 王萍 梁亮 胡雪芳 张志民 裴海生 ZHAI Xiaona;HU Longbiao;LI Yuanyuan;ZHOU Xinqun;WANG Ping;LIANG Liang;HU Xuefang;ZHANG Zhimin;PEI Haisheng(Academy of Agricultural Planning and Engineering,MARA,Key Laboratory of Agro-Products Primary Processing,Ministry of Agriculture and Rural Affairs,Beijing 100125,China)

机构地区：[1]农业农村部规划设计研究院,农业农村部农产品产地初加工重点实验室,北京100125

出　　处：《食品工业科技》2024年第12期189-197,共9页Science and Technology of Food Industry

基　　金：农规英才计划(QNYC-2021-10)。

摘　　要：本文引入超微粉碎前处理技术,以菜籽饼超微粉为原料直接酶解制备活性肽,利用单因素实验和响应面试验优化其制备工艺,酶解液经膜分离和Sephadex G-15凝胶分离后获得活性肽,并对其抗氧化活性和结构进行分析研究。结果表明,菜籽饼活性肽的最佳制备条件为:超微粉碎处理30 min、料液比1:20、加酶量6300 U/g、酶解pH2.94、酶解温度55℃、酶解时间2.25 h,此条件下活性肽得率为83.28%;菜籽饼酶解液中分子量低于1000 Da的F-2组分具有较好的抗氧化活性,1 mg/mL下对ABTS+自由基的清除率为71.62%,从中鉴定出PF、SF、TF、IAIPF和PLDLL五条预测活性最高的肽段。本研究结果可为以菜籽饼为原料直接开发活性肽提供一定的理论依据。To prepare bioactive peptides from rapeseed cake directly,the superfine grinding technology was used,and the resulting rapeseed cake superfine powder was used as the material.Single-factor experiments and response surface analysis were used to optimize the preparation extraction.The active peptides were purified by membrane separation and Sephadex G-15 membrane separation,and their antioxidant activity and structure were evaluated.The results showed that the optimal preparation conditions were as follows:grinding time 30 min,solid-to-liquid ratio 1:20(g/mL),pH2.94,enzyme dosage 6300 U/g,temperature 55℃,and enzymatic hydrolysis time 2.25 h.Under these conditions,the yield of peptides could reach 83.28%.The F-2 fraction from hydrolysates with molecular mass less than 1000 Da showed good antioxidant activity,with an ABTS+free radical eliminating rate of 71.62%at a concentration of 1 mg/mL.Furthermore,five peptides were identified,namely PF,SF,TF,IAIPF,and PLDLL which were predicted to have much higher activity.Overall,the results of this study can provide a theoretical basis for the exploration of bioactive peptides directly from rapeseed cakes.

关 键 词：菜籽饼 超微粉碎 风味蛋白酶 活性肽 抗氧化活性 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]