戊糖片球菌的抑菌与共凝聚能力及其对水体中副溶血性弧菌的净化效应  

Antibacterial and Coaggregation Activities of Pediococcus pentosaceus and Its Putificatiion Effect on Vibrio parahaemolyticus-contaminated Water

作　　者：杨振泉[1,2] 高璐 靳彩娟[1] 王晓霖 方维明[1] 顾瑞霞[1,2] 

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225127 [2]江苏省乳品生物技术与安全控制重点实验室,江苏扬州225127

出　　处：《现代食品科技》2015年第8期47-52,共6页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31371806);苏北科技发展计划项目(No.BC2013438);江苏省重点实验室开放课题;江苏省自然科学基金项目(No.BK2011448)

摘　　要：研究了7株戊糖片球菌对副溶血性弧菌的抑菌特性和共凝聚能力,探讨了不同抑菌和凝聚特性的戊糖片球菌的细胞形态特征及其对水体中副溶血性弧菌的影响。结果表明7株戊糖片球菌对副溶血性弧菌的抑制作用是同p H值乳酸的1.1~1.3倍,抑菌活性对过氧化氢酶、蛋白酶以及加热处理不敏感。戊糖片球菌对副溶血性弧菌的2 h共凝聚率在14.8~37.6%之间,具有显著菌株差异性,其中菌株F28-8达到37.6%,显著高于其它菌株(P<0.01)。扫描电镜结果显示菌株F28-8具有独特的细胞表面结构和粘连模式。戊糖片球菌对水体中副溶血性弧菌具有促凝聚和减菌作用,副溶血性弧菌悬液经过F28-8处理60 h,上层悬液中的可培养细胞浓度比未经处理的对照组降低了3.1 Log10 CFU/m L,显著高于低凝聚性菌株H13(P<0.01),而凝聚物中可培养细胞比对照组降低了1.6 Log10CFU/m L,与H13处理无显著差异(P>0.05)。Coaggregation and antibacterial activity of seven P. pentosaceus strains against Vibrio parahaemolyticus ATCC33847 were evaluated. And their cellular morphological characteristics as well as impact on o Vibrio parahaemolyticus ATCC33847 in contaminated water, were explored. The results showed that the inhibitory effect of the seven P. pentosaceus strains tested was 1.1 to 1.3 times that of lactic acid at the same p H, while the antibacterial activity was resistant to catalase, proteinase, and heat treatments. The 2-h coaggregation rate of the seven isolates against V. parahaemolyticus varied between 14.8% and 37.6%, showing a significant inter-strain difference. Among the seven isolates, the coaggregation activity of F28-8 reached 37.6%, which was significantly higher than those of other strains(p < 0.01). The results from scanning electron microscopy(SEM) showed that strain F28-8 had a unique cell surface structure and cell adhesion pattern. P. pentosaceus also played a role in promoting aggregation and reducing numbers of viable V. parahaemolyticus in water. After a suspension of V. parahaemolyticus was treated with strain F28-8 for 60 h, the concentration of culturable cells in the upper suspension layer was lowered by 3.1 Log10 CFU/m L compared to that of the untreated group, which was significantly higher than that of strain H13, which showed low coaggregation activity(2.5 log10 CFU/m L). On the other hand, the concentration of culturable cells in the coaggregates was 1.6 log10 CFU/m L, which was lower than that of the control group and showed no significant change with H13 treatment(p > 0.05).

关 键 词：戊糖片球菌 抑菌能力 共凝聚能力 副溶血性弧菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]