芸豆皮多酚制备、纯化、鉴定及抗油脂氧化作用研究  

Preparation,Purification,Identification and Anti-lipid Oxidation of Kidney Bean Skin Polyphenols

作　　者：孙立兰 王坤 张友良 王若坤 左锋[1,3] SUN Lilan;WANG Kun;ZHANG Youliang;WANG Ruokun;ZUO Feng(College of Food Science,Heilongjiang Bayi Agricultural Univercity,National Coarse Cereals Engineering Research Center,Daqing 163319,China;Daqing Old Street Ji Agricultural Sideline Products Co.,Ltd.,Daqing 163319,China;Engineering Research Center for Processing and Utilization of Grain By-products,Ministry of Education,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学食品学院,国家杂粮工程技术中心,黑龙江大庆163319 [2]大庆老街基农副产品有限公司,黑龙江大庆163319 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319

出　　处：《食品工业科技》2025年第2期292-300,共9页Science and Technology of Food Industry

基　　金：黑龙江省重点研发计划项目(GA21B011);校引进人才启动计划(XDB-2017-13)。

摘　　要：为了探究紫花芸豆皮多酚的制备纯化工艺及抗油脂氧化作用,本研究以紫花芸豆皮为原料,采用单因素实验考察了提取溶剂浓度、提取温度、提取时间、料液比和提取次数对芸豆皮多酚提取量的影响;利用HPD600大孔树脂对芸豆皮多酚粗提液进行了纯化,并采用液相色谱质谱联用仪分析酚类物质组成;以过氧化值为指标利用Arrhenius方程建立花生油的货架期预测模型。结果表明,乙醇浓度60%、提取温度70℃、提取时间40 min、液料比1:60(g:mL)、提取次数4次为芸豆皮多酚的最佳提取条件,此时芸豆皮多酚提取量为0.18±0.49 g/g;HPD600大孔树脂对紫花芸豆皮多酚的静态吸附容量、解吸率最大,适合分离纯化紫花芸豆皮多酚,最佳纯化工艺为上样流速1.0 mL/min、洗脱剂乙醇浓度60%和洗脱流速1.0 mL/min,此时芸豆皮多酚纯度为54.68%±1.97%;芸豆皮中含有19种单体酚,其中儿茶素含量最高,为425.952±7.652μg/g;添加芸豆皮多酚或抗氧化剂能有效延缓食用油过氧化值升高,通过模型推算多酚-维生素C复配的花生油在20℃条件下的货架期最长可达193.4 d,对照组货架期为150.3 d,与实测值最大相对误差为8.7%,可准确预测该食用油的货架期。综上,芸豆皮多酚具有较强的抗氧化性,具有安全的天然食品抗氧化剂的潜在用途。In order to explore the preparation and purification process of polyphenols from purple flower kidney bean skins and their anti-lipid oxidation effect,this study investigated the effects of solvent concentration,extraction temperature,extraction time,solid-liquid ratio and number of extraction on extraction amount of polyphenols from purple flower kidney bean skins by single factor experiments.Purified the crude extraction with HPD600 macroporous resin,and analyzed the phenolic composition using liquid chromatography-mass spectrometer.Shelf life predication model of peanut oil was established with peroxide value as the indicator using the Arrhenius equation.The experimental results showed that the optimal extraction conditions for kidney bean skin polyphenols were:ethanol concentration of 60%,extraction temperature of 70℃,extraction time of 40 min,solid-to-liquid ratio of 1:60(g:mL),and 4 times of extraction,under these conditions,the extraction amount of polyphenols was 0.18±0.49 g/g.HPD600 had the highest static adsorption capacity and desorption rate,making it suitable for purifying polyphenols from purple kidney bean skins.The optimal purification conditions were:loading flow rate of 1.0 mL/min,ethanol concentration of 60%,elution flow rate of 1.0 mL/min,and the maximum purity of polyphenols was 54.68%±1.97%.Kidney bean skins contained 19 types of monomer phenols,with the highest catechin content at 425.952±7.652µg/g.Adding kidney bean skin polyphenols or antioxidants could effectively delay the increase in peroxide value of edible oil.Using a model,it was calculated that peanut oil mixed with polyphenols-vitamin C had a maximum shelf life of 193.4 d at 20℃,control group was 150.3 d,with a maximum relative error of 8.7%compared to measured values,accurately predicting the shelf life of the edible oil.In conclusion,kidney bean skin polyphenols had strong antioxidant properties and potential as a safe natural food antioxidant.

关 键 词：芸豆皮 多酚 分离鉴定 抗油脂氧化 货架期 

分 类 号：TS209[轻工技术与工程—食品科学]