鹿茸菇可溶性膳食纤维生物活性及结构特征分析  

Bioactivity and Structural Characterization of Soluble Dietary Fiber from Lyophyllum decastes

作　　者：何姗珊 刘利平 刘艳芳[2] 冯杰[2] 王光强[1] 汪雯翰[2] 张劲松[2] HE Shanshan;LIU Liping;LIU Yanfang;FENG Jie;WANG Guangqiang;WANG Wenhan;ZHANG Jinsong(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Shanghai 201403,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点研究室,国家食用菌工程技术研究中心,国家食用菌加工技术研发中心,上海201403

出　　处：《食品科学》2025年第9期39-47,共9页Food Science

基　　金：上海市现代农业产业技术体系(沪农科产字(2024)第9号);上海市农业科学院卓越团队建设计划(沪农科卓(2022)003)。

摘　　要：食用菌来源可溶性膳食纤维(soluble dietary fiber,SDF)具有良好的生物活性,为开发利用食用菌中的SDF,本研究以不同种食用菌为研究对象,采用复合酶解法水解不同食用菌并测定其中的SDF含量,筛选出高SDF含量的食用菌,评价其体外抗氧化活性、亚硝酸盐吸附、体外降糖(葡萄糖吸附能力、α-淀粉酶抑制活性、α-葡萄糖苷酶抑制活性)和降脂活性(胆固醇酯酶抑制活性、胰脂肪酶抑制活性和胆固醇胶束溶解度吸附能力),最终选出综合生物活性最好的鹿茸菇进行结构特征分析。单糖分析结果表明,鹿茸菇SDF由葡萄糖、半乳糖、甘露糖和葡萄糖醛酸等组成;傅里叶变换红外光谱分析结果表明,鹿茸菇SDF具有多糖的特征吸收峰;扫描电子显微镜分析结果表明,鹿茸菇SDF具有明显的孔隙结构。本研究结果表明鹿茸菇SDF是一种可开发降脂或降血糖功能性食品的优质原料。Soluble dietary fiber(SDF)from edible mushrooms has good biological activity.In order to develop and utilize SDF from edible mushrooms,the present study determined the SDF contents in different edible mushrooms by sequential enzymatic hydrolysis.SDF-rich mushrooms were selected and evaluated for their in vitro antioxidant activity,nitrite adsorption capacity,in vitro glucose-lowering(glucose adsorption capacity,α-amylase inhibitory andα-glucosidase inhibitory activity)and lipid-lowering(cholesterol esterase,pancreatic lipase,and cholesterol micelle solubility inhibitory activity)activities.Finally,SDF from Lyophyllum decastes,which had the best overall bioactivity among all tested species,was selected for structural characteristic analysis.Monosaccharide analysis showed that SDF from L.decastes consisted of glucose,galactose,mannose and glucuronic acid.Fourier transform infrared(FTIR)spectroscopy showed that the SDF exhibited characteristic absorption peaks of polysaccharides.Scanning electron microscopy(SEM)showed that it possessed an obvious porous structure.The results of this study indicate that the SDF from L.decastes is an excellent ingredient for functional foods with hypoglycemic and hypolipidemic activity.

关 键 词：可溶性膳食纤维 鹿茸菇 降糖活性 降脂活性 抗氧化活性 结构特征 

分 类 号：TS201.4[轻工技术与工程—食品科学]