具有生物保护潜力的耐低温乳酸菌筛选及其抑菌特性  

Selection and Bacteriostatic Properties of Cryotolerant Lactic Acid Bacteria with Bioprotective Potential

作　　者：杜文静 王强 陈倩[1] 刘骞[1] 孔保华[1] DU Wenjing;WANG Qiang;CHEN Qian;LIU Qian;KONG Baohua(College of Food Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2025年第9期121-129,共9页Food Science

基　　金：国家自然科学基金面上项目(32372373);国家自然科学基金联合基金重点项目(U22A20547)。

摘　　要：本研究筛选具有生物保护潜力的耐低温乳酸菌菌株,并探究其在低温条件下的适应性及抑菌特性。结果表明,在4℃和7℃培养温度下,与清酒广布乳杆菌(Latilactobacillus sakei)B2相比,乳酸片球菌(Pediococcus acidilactici)L1和植物乳植杆菌(Lactiplantibacillus plantarum)HG1-1具有较高的低温耐受能力,在低温条件下仍具有较高的超氧化物歧化酶活力和蛋白酶活力,且菌株形态仍然保持完整。筛选出的2株菌能显著抑制肉制品中常见的腐败菌表皮葡萄球菌(Staphylococcus epidermidis)、鲍氏不动杆菌(Acinetobacter baumannii)和热杀索丝菌(Brochothrix thermosphacta)的生长,其抑菌能力显著高于商业保护菌L.sakei B2。这2种菌株的胞外代谢产物经85℃热处理后仍具有一定的抑菌活性,经胰蛋白酶处理其抑菌能力会显著降低。此外,这2种菌株的胞外代谢产物的抑菌活性随pH值升高而降低,当pH 7时无抑菌性。综上所述,P.acidilactici L1和L.plantarum HG1-1在低温条件下有一定的抑菌能力,具有作为低温肉制品生物保护剂的潜力。This study aimed to select cryotolerant lactic acid bacteria with bioprotective potential and to investigate their low-temperature adaptability and bacteriostatic properties.The results demonstrated that Pediococcus acidilactici L1 and Lactiplantibacillus plantarum HG1-1 exhibited a higher tolerance to low temperatures(4 and 7℃)when compared with Latilactobacillus sakei B2.In addition,they maintained high activities of superoxide dismutase and protease,as well as good morphological integrity.These two strains significantly inhibited the growth of common spoilage bacteria(Staphylococcus epidermidis,Acinetobacter baumannii,and Brochothrix thermosphacta)in meat products and their inhibitory capacity was substantially higher than that of the commercial strain L.sakei B2.The extracellular metabolites of the two strains retained antibacterial activity after heat treatment at 85℃.Trypsin treatment significantly decreased the bacteriostatic activity of the metabolites.Moreover,the bacteriostatic activity of both strains decreased as the pH level increased,disappearing at pH 7.In conclusion,both P.acidilactici L1 and L.plantarum HG1-1 have bacteriostatic activity under low-temperature conditions,showing potential as bioprotectants for low-temperature meat products.

关 键 词：乳酸菌 低温适应性 抑菌性 生物保护菌 腐败菌 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]