产转谷氨酰胺酶链霉菌的发酵罐生产工艺研究  

Optimization of Fermentation Process for Streptomyces Transglutaminase Production

作　　者：蔡慧农[1] 王灼维[2] 杨秋明[1] 郭兴要[2] 刘新征[2] 韩兆鹏[2] 王璋[2] 

机构地区：[1]集美大学生物工程学院 [2]中国食品发酵工业研究院,北京100027

出　　处：《食品与发酵工业》2003年第8期13-18,共6页Food and Fermentation Industries

摘　　要：针对研究室分离筛选的链霉菌 (Streptomycessp )WZFF W 12 varMN 3 5发酵生产微生物转谷氨酰胺酶 (MTG) ,首先采用自行设计的 2L小型生化反应器 ,研究以多价胨为主的有机氮源的影响作用 ,并在此基础上逐级扩大发酵罐规模 ,以 8L小型罐及 2 0～ 2 0 0L发酵罐组合并利用在线监控手段直接监测分析环境条件对MTG发酵过程中菌体生长和产酶的影响 ,进一步确定培养条件。研究结果表明 ,以 2 0 0L发酵罐发酵生产MTG时采用两级种子培养 ,氮源采用多价胨和豆饼粉 ,发酵过程中在线控制通气量和搅拌速度分别为 0 9～ 1 1vvm和 3 5 0～ 45 0r/min等优化条件最有利于菌体持续稳定生产MTG ,当发酵至 72h时酶活达到最高 ,在 2The effects of complex nitrogen sources mainly containing multi peptone in fermentation broth on the production of microbial transglutaminase (MTG) by a stable mutant, Streptomyces sp WZFF W 12 var MN 35 screened from the complex mutation treatments of soil microbial isolates were first investigated by using 2?L mini bioreactor we designed and equipped, and the culture conditions were established The fermentation scale was further piloted up to a 8L jar fermenter and a 20～200?L fermenter complex with the utilization of an on line control aid to supervise and analysis the effects of environmental parameters Through the batch fermentation tests a suitable technique for MTG production with the 200L fermenter was concluded mainly as the use of 1 0% multi peptone and 1 0% crude soybean powder for the nitrogen source to cut the costs of raw materials, and the stepwise control of aeration rate and agitation speed to be 0 9～1 1 vvm and 350～450 ?r/min, respectively, by on line control system during fermentation process to enhance the MTG productivity following a two step seed culture Through carrying out this effective operation of these optimal conditions, the batch fermentation with the strain MN 35 went on smoothly and gave repeatable MTG productions with stable enzyme activitives higher than 2 15?u/mL

关 键 词：转谷氨酰胺酶 链霉菌 发酵罐 发酵生产 多价胨 在线监控 

分 类 号：TQ920[轻工技术与工程—发酵工程]