响应面法优化载儿茶素叶酸偶联壳聚糖纳米粒的制备工艺  

Optimization of Preparation of Catechin-Loaded Folate-Conjugated Chitosan Nanoparticles by Response Surface Analysis

作　　者：刘步云[1] 王永丽[2] 于奇建 刘芳[1] 臧金红 金铃和 李大鹏[1] 李锋[1] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2014年第14期46-52,共7页Food Science

基　　金：国家级大学生创新创业训练计划项目(201210434006)

摘　　要：采用响应面法对载儿茶素叶酸偶联壳聚糖纳米粒的制备工艺进行优化。利用叶酸活性酯与壳聚糖酰化反应制备叶酸偶联壳聚糖。通过单因素和响应面试验,以儿茶素包封率为考察指标,采用分子自组装原理制备载儿茶素叶酸偶联壳聚糖纳米粒并进行工艺优化。结果表明,最佳制备工艺条件为:儿茶素与叶酸偶联壳聚糖质量比9∶20、叶酸偶联壳聚糖与三聚磷酸钠质量比3.93∶1、反应溶液pH 5.15、搅拌时间32.35 min。在此条件下,儿茶素包封率为23.45%,与理论值23.90%接近。透射电镜图表明所制纳米粒大小均匀,接近于规则球形,粒径分布在200~500 nm范围内。利用响应面法优化载儿茶素叶酸偶联壳聚糖纳米粒的制备工艺是可行的。The preparation process for catechin-loaded folate-conjugated chitosan nanoparticles(Catechin-FA-CS) was optimized using response surface methodology. Folate chitosan conjugates(FA-CS) were synthesized based on chemical linking of carboxylic group of active folate ester with amino group of chitosan. Then,Catechin-FA-CS nanoparticles were prepared by self-assembling. The optimum process conditions were as follows: mass ratio of catechin to FA-CS 9:20,mass ratio of FA-CS to sodium tripolyphosphate 3.93:1,pH 5.15,and stirring time 32.35 min. Under these conditions,the experimental catachin entrapment efficiency was 23.45%,which was close to the predicted value of 23.90%. Transmission electron micrograph showed that catechin-FA-CS particles were well dispersed as a near-spherical shape within the range of 200–500 nm. These results showed that response surface methodology is applicable for the optimization of preparation of Catechin-FA-CS nanoparticles.

关 键 词：儿茶素 叶酸 壳聚糖 纳米粒 响应面 

分 类 号：TQ461[化学工程—制药化工] TQ914.1