河蚬中水溶性蛋白的提取及其抗氧化性质  

Water-Soluble Proteins from Corbicula fluminea: Optimization of Ultrasound-Assisted Extraction Process by Response Surface Analysis and Antioxidant Potential

作　　者：杨玉娈 袁美兰[1] 陈丽丽[1] 白春清[1] 赵利[1] 石岭[1] 苏伟[1] 

机构地区：[1]江西科技师范大学生命科学学院国家淡水鱼加工技术研发分中心(南昌),江西南昌330013

出　　处：《食品科学》2015年第6期96-102,共7页Food Science

基　　金：南昌市农业科技支撑计划项目(洪财企[2012]80号);江西省教育厅科技重点项目(GJJ12582)

摘　　要：研究超声波辅助法提取河蚬水溶性蛋白的工艺。以水溶蛋白的提取率为考察指标,以料液比、提取时间、提取温度和超声功率为考察因素,采用响应面法对河蚬水溶性蛋白提取工艺条件进行优化设计,并对其进行抗氧化研究。结果表明:最佳河蚬水溶性蛋白提取工艺条件为料液比1∶20、提取时间23 min、提取温度48℃、超声功率920 W,此条件下河蚬水溶性蛋白提取率达到最大值,为67.70%。其中,提取时间和超声功率对河蚬水溶蛋白提取率的影响显著。超声波的空化和振动作用可以促进细胞的破碎,使目标产物与溶剂充分混合,增大提取率,缩短提取时间,对河蚬中水溶性蛋白的提取具有很大意义。河蚬提取液具有较强的清除1,1-二苯基-2-三硝基苯肼自由基、超氧阴离子自由基的能力和较强的铁离子还原能力,而清除羟自由基的能力较弱。Ultrasound-assisted extraction was adopted to extract water-soluble proteins(WSP) from Corbicula fluminea. The optimization of four extraction parameters including solid to liquid ratio, extraction time, extraction temperature, and ultrasound power for the enhanced yield of water-soluble proteins was carried out using Box-Behnken design(BBD) and response surface methodology(RSM). Moreover, the antioxidant potential of WSPs from Corbicula fluminea was investigated. The optimal extraction conditions that provided maximum WSP yield(67.70%) were determined as follows: solid to liquid ratio, 1:20; extraction time, 23 min; extraction temperature, 48 ℃; and ultrasonic power, 920 W. The analysis of variance(ANOVA) showed that the effects of extraction time and ultrasound power on WSP yield were significant. The cells were broken more significantly by ultrasonic cavitation and vibration to fully expose the target product to the solvent, accordingly increasing the extraction efficiency and shortening the extraction time. Thus, the application of ultrasonic is of great significance for water-soluble protein extraction. The scavenging activity against 1,1-diphenyl-2-picrylhydrazyl(DPPH) and superoxide anion radicals and ferric ion reducing power of the extracts were strong, but the hydroxyl radical scavenging activity was weak.

关 键 词：河蚬 水溶性蛋白 超声波 提取 抗氧化 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]