supported by grants from National Twelfth Five-Year Plan for Science&Technology Support of China(2014BAI13B03);National Natural Science Foundation of China(81460248,81260457,81271899,31272592,81071320);Inner Mongolia Natural Science Funds(2013MS1138,2012MS1121,2011MS1110);Beijing Natural Science Foundation(6122030,7132153);Inner Mongolia Science&Technology Plan(20120101,20120402,20110502);the National Key Program for Infectious Diseases of China(2013ZX10004-203,2013ZX10004-217-002,2013ZX10004805-006).
Brucellosis is a worldwide zoonosis.Vaccination is the most efficient means to prevent and control brucellosis.The current licensed attenuated vaccines for animal use were developed by sequential passage in nonnatural...