supported by the National Natural Science Foundation of China (Grant Nos. 30830006 and 31000045);the National High Technology Research and Development Program of China (Grant No. 2007AA021305);Major Scientific and Technological Project for the Creation of Significant New Drugs (Grant No. 2009ZXJ09001-034);Major Project for Genetically Modified Organism Breeding (Grant No. 2009ZX08009-075B);Application of Nuclear Techniques in Agriculture from Ministry of Agriculture of China (Grant No. 200803034) to Yue-jin Hua;the 2009 Zhejiang Innovation Program for Graduates
The β-subunit of RNA polymerase, which is involved in rifampin binding, is highly conserved among prokaryotes, and Rile mutants detected in many bacteria are the result of amino acid changes. Spontaneous rifampin res...