The National Natural Science Foundation of China(No.60071001);China Postdoctoral Science Foundation(No.2002);Trans-Century Training Programme Foundation for the Talents by theState Education Commission(No.[2004]527);the Foundation of the 135?Key Laboratory of Jiangsu Province(No.SK200205);the HighTechnology Research Plan of Jiangsu Province(No.BG2003033,BG2001010).
Mutations in cardiac troponin I (cTnI) gene were assessed based on gene-chip technology.Special probes were designed to fabricate the low-density gene-chip,which could detect the mutations in exons 3,5,7,and 8 of th...
To quantitatively detect hypermethylation and to analyze methylation pattern, a methylation-specific oligonucleotide microarray for quantitative analysis was developed. The method used bisulfite-modified DNA as a temp...
The phosphorous amide approach is of the highest coupling efficiency among the DNA in situ synthesis approaches at present. The strict anhydrous condition is required in the process of DNA in situ synthesis, and the D...
Microporous polyamide-6 membranes were hydrolyzed in 0.01 mol/L NaOH/CH 3OH medium by refluxing for about 36 h. By investigating the UV spectra, the hydrolyzing conditions were optimized. A pH value of 12 and reaction...