The interaction of Cu(Ⅱ) and human serum albumin (HSA) or bovine serum albumin (BSA) at physiological pH is studied by equilibrium dialysis. The successive stability constants are obtained by non-linear least square ...
In the study of mechanism of hormone action, the identification and localization of hormone receptors were important research fields. Cytokinin(CTK)-binding proteins, have been found from some cell fractions of severa...
In recent years the reconstitution technique has been widely used in the study on the structure and function of membrane proteins and its interaction with lipids. We have reported that during the reconstitution of por...
Hemoglobin M (HbM) is one kind of abnormal hemoglobin which leads to the genetic methemoglobin disease. So far five hemoglobins M have been already reported. The hemoglobin M we studied in this note was found in Shang...
Previous studies have reported that, during reconstitution of pig heart mitochondrial H+-ATPase in soybean phospholipid liposomes by cholate dialysis method, 1mM Mg2+ in the dialysis medium greatly enhances the recons...
As to the action of human serum albumin (HSA) with rare earth, no reports have been made yet except that Section et al. have investigated the binding of 40Sc, 90Y, 140La to HSA by using equilibrium dialysis. In this...
It was reported that activities of the mitochondrial H+-ATPase reconstituted on liposomes, performed by cholate dialysis method in the presence of 1 mM Mg2+ in the dialysis medium, were markedly increased and the resu...
In one of our previous papers, we reported that during reeonstitution of pigheart H+- ATPase with soybean phspholipid liposome, the presence of Mg2+ could greatly enhance the activities of the reconstituted system (32...
In recent years the reconstitution of vesicles capable of ATP-driven energy transformation has been carried out in many laboratories[1-5], but the results are not always easily reproducible. It seems to be very import...