In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3% nucleo- tide sequence similarity with published pac1 in GenBank. In pET-5α expression system, the expres- sion product of cloned pac1 in E...
The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay th...
Great progress has been made in recent years in studies on the mechanism of Agrobacterium-mediated transformation and its application. Many details of the key molecular events within the bacterial cells involved in T-...
Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-in-ducible promoter has been introduced into Brassica napus by coculture with Agrobacterium tumefaciens and laser microbeam puncture respective...
Virulence (vir) genes of Ti plasmids code for a specialized conjugation system that mediates the mobilization and transfer of T-DNA from Agrobacterium to the genome of higher plants. This system is activated by a numb...
Project supported by the Rockefeller Foundation,Academic Funds for Young Researchers of Higher Education Institutions in shanghai;the Science Committee of Shanghai.
Since 1988 the substantial development of rice transformation techniques has made it possible to introduce cloned gene into rice protoplast (by electroporation and/or PEG method) or into intact cell (by particle bomba...
Present address: Biotechnology Research Center, Zhongshan University, Guangzhou 510275, PRC
The development of plant gene engineering requires a simple, rapid and efficient system for genetic transformation. It is recently demonstrated that certain compounds, secreted by host plant cells, are capable of elic...
Solanum nigrum is a herb useful for medicine and can also be used as a receptor system in gene transfer. Teratomas can be obtained in Solanum nigrum by infection of Agrobacterium tumefaciens and T-DNA plantlets differ...
The problems of introducing foreign DNA into plant cells and of integrating and expressing foreign genes in plant cells must be solved in plant genetic engineering. As reported before, Ti plasmid of Agrobacterium tume...