AGROBACTERIUM

作品数:129被引量:267H指数:9
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相关领域:农业科学生物学更多>>
相关作者:郭子琦陈章良王莉江苑华毅明小天更多>>
相关机构:中国农业大学江南大学华中农业大学四川农业大学更多>>
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相关基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划河北省自然科学基金更多>>
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Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV被引量:5
《Chinese Science Bulletin》2006年第19期2362-2368,共7页YAN Fei ZHENG Yinying ZHANG Wenwei XIAO Hong LI Shifang CHENG Zhuomin 
In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3% nucleo- tide sequence similarity with published pac1 in GenBank. In pET-5α expression system, the expres- sion product of cloned pac1 in E...
关键词:转基因小麦 pac1 土壤杆菌 抗病毒素 大麦黄矮病 
Resistance to rice blast (Pyricularia oryzae) caused by the expression of trichosanthin gene in transgenic rice plants transferred through agrobacterium method被引量:12
《Chinese Science Bulletin》2000年第19期1774-1778,共5页Xiaotian Ming Lijiang Wang Chengcai An Huayi Yuan Zhangliang Chen 
The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay th...
关键词:TRICHOSANTHIN antifungi AGROBACTERIUM rice. 
Agrobacterium-mediated transformation: state of the art and future prospect被引量:1
《Chinese Science Bulletin》2000年第17期1537-1546,共10页Wei Li Guangqin Guo Guochang Zheng 
Great progress has been made in recent years in studies on the mechanism of Agrobacterium-mediated transformation and its application. Many details of the key molecular events within the bacterial cells involved in T-...
关键词:AGROBACTERIUM -mediated TRANSFORMATION FUNGI MONOCOTS large DNA fragment. 
Introduction of pokeweed antiviral protein cDNA into Brassica napus and acquisition of transgenic plants resistant to viruses被引量:3
《Chinese Science Bulletin》1999年第8期701-704,共4页ZHANG Haiyan , TIAN Yingchuan , ZHOU Yihua , DANG Benyuan LAN Haiyan , SONG Guiying , WANG Lanlan , LIU Guizhen , ZHANG Lihua and CHEN ZhenghuaInstitute of Genetics , Chinese Academy of Sciences , Beijing 100101, China Institute of Microbiology , Chinese Academy of Sciences , Beijing 100080, China 
Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-in-ducible promoter has been introduced into Brassica napus by coculture with Agrobacterium tumefaciens and laser microbeam puncture respective...
关键词:pokeweed antiviral protein CDNA wound-inducible promoter AGROBACTERIUM TUMEFACIENS laser MICROBEAM puncture. 
Identification of a novel signal for activation of Ti plasmid- encoded vir genes from rice (Oryza sativa L.)
《Chinese Science Bulletin》1995年第21期1824-1828,共5页施骏 许耀 刘吉开 尹中朝 李宝健 
the National Natural Science Foundation of China.
Virulence (vir) genes of Ti plasmids code for a specialized conjugation system that mediates the mobilization and transfer of T-DNA from Agrobacterium to the genome of higher plants. This system is activated by a numb...
关键词:RICE SIGNAL molecule AGROBACTERIUM tumefaaences Ti PLASMID virulence. 
Transformation of Rice (Oryza sativa, L.) by Agrobacteriittn tumefaciens
《Chinese Science Bulletin》1994年第17期1473-1476,共4页李瑶 沈大棱 潘小舟 任大明 秦瑞珍 叶鸣明 刘祖洞 
Project supported by the Rockefeller Foundation,Academic Funds for Young Researchers of Higher Education Institutions in shanghai;the Science Committee of Shanghai.
Since 1988 the substantial development of rice transformation techniques has made it possible to introduce cloned gene into rice protoplast (by electroporation and/or PEG method) or into intact cell (by particle bomba...
关键词:AGROBACTERIUM tumefadens ANTI-BACTERIAL peptide RICE adventitious BUD transformation. 
PHENOLIC COMPOUNDS PROMOTE HIGH EFFICIENCY TRANSFORMATION OF PLANT EXPLANTS in vitro BY Agrobacterium tumefaciens被引量:1
《Chinese Science Bulletin》1989年第22期1902-1907,共6页许耀 贾敬芬 郑国锠 
Present address: Biotechnology Research Center, Zhongshan University, Guangzhou 510275, PRC
The development of plant gene engineering requires a simple, rapid and efficient system for genetic transformation. It is recently demonstrated that certain compounds, secreted by host plant cells, are capable of elic...
关键词:pbenollc compounds Vir-region activation in VITRO transformation T-DNA transfer AGROBACTERIUM TUMEFACIENS 
PLANT REGENERATION AND DIFFERENTIATION OF ADVENTIVE BUDS FROM T-DNA-CALLUSES IN Solanum nigrum
《Chinese Science Bulletin》1986年第6期409-412,共4页蒋兴邨 邵启全 王大桢 
Solanum nigrum is a herb useful for medicine and can also be used as a receptor system in gene transfer. Teratomas can be obtained in Solanum nigrum by infection of Agrobacterium tumefaciens and T-DNA plantlets differ...
关键词:SOLANUM DNA differentiated AGROBACTERIUM sterile shoot night CALLUS supplemented nutrition 
T-DNA TRANSFER AND TERATOME INDUCTION ON Brassica oleracea L
《Chinese Science Bulletin》1985年第6期826-828,共3页蒋兴邨 邵启全 周德成 
The problems of introducing foreign DNA into plant cells and of integrating and expressing foreign genes in plant cells must be solved in plant genetic engineering. As reported before, Ti plasmid of Agrobacterium tume...
关键词:PLASMID EXPRESSING DNA AGROBACTERIUM introducing integrating AUXIN ARGININE TRANSFERRED verified 
A MODIFIED METHOD FOR ASSAY OF OPINE
《Chinese Science Bulletin》1984年第5期709-710,共2页陈劲春 林忠平 
The tumor-inducing plasmid (pTi) of Agrobacterium tumefaciens is a most promising vector system in the genetic engineering of
关键词:PLASMID INDUCING AGROBACTERIUM REAGENT qualitative DNA dissolved referred 
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