CELL-FREE

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Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract
《Chinese Science Bulletin》2001年第17期1449-1453,共5页Zhigang Lu Wei Yang Qi Cao Wei Tao Jiancheng Hu Zhonghe Zhai 
the National Basic Research Project (Grant No. G1999053905).
When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, l...
关键词：XENOPUS LAEVIS APOPTOSIS IXAD XAD DNA LADDER CYTOCHROME c CELL-FREE system. 
Construction of a cell-free system using extracts from apoptotic plant cells被引量：1
《Chinese Science Bulletin》1999年第16期1494-1497,共4页Jun Zhou Haoming Chen Xiaofang Jiang Haizhen Zhu Yaoren Dai 
To study the mechanism of plant apoptosis, a cell-free system has been developed using the extracts prepared from tobacco cells that become committed to apoptosis induced by heat shock treatment. Tobacco nuclei have d...
关键词：APOPTOSIS CELL-FREE system PROTEASE PROTEASE inhibitor. 
Apoptosis of carrot nuclei in in vitro system induced by cytochrome c
《Chinese Science Bulletin》1999年第16期1497-1502,共6页Yun Zhao Yingli Sun Zhengfan Jiang Zhonghe Zhai 
The apoptosis of carrot nuclei induced in cytosol of carrot cells by cytochrome c is reported. This kind of cell-free apoptosis system of plant, CS-100, is an efficient In vitro inducing system. Several typical charac...
关键词：APOPTOSIS CYTOCHROME c CYTOSOL CELL-FREE system CARROT nuclei. 
Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis被引量：4
《Chinese Science Bulletin》1998年第6期522-526,共5页Shan Zhu Zhengfan Jiang Bo Zhang Zhigang Lu Zhonghe Zhai 
A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which ...
关键词：APOPTOSIS DNASE CELL-FREE system XENOPUS EGG EXTRACTS 
Effect of high pressure on activity of rabbit reticulocyte cell-free protein-synthesizing system
《Chinese Science Bulletin》1995年第11期935-938,共4页金由辛 阮康成 包俊茹 龚佩娟 郑乐 王德宝 
Studies so far indicate that many processes in the interactions between biomacromole-cules are accompanied by a change in occupied volume. For instance, the association ofprotein subunits into oligomer and the formati...
关键词：CELL-FREE protein-synthesizing SYSTEM EFFECT of high pressure. 
Nuclear Matrix in the Nucleus Assembled in vitro
《Chinese Science Bulletin》1993年第18期1567-1571,共5页蔡树涛 翟中和 
Project supported by the National Natural Science Foundation of China.
1 Introduction Nuclear assembly occurs universally in cell life, such as postmitotic or postmeiotic nuclear assembly. It is a good model for studying the organization and function of nucleus. The organism or even sing...
关键词：NUCLEAR RECONSTITUTION CELL-FREE system NUCLEAR matrix NUCLEAR LAMINA 
ENHANCEMENT OF BRAIN AND SPLEEN LYMPHOCYTE PROTEIN SYNTHESIS ACTIVITIES DURING COLD ADAPTATION IN RATS
《Chinese Science Bulletin》1991年第9期752-757,共6页徐扬 杨泽田 苏成芝 
In cold surroundings, in both plants and animals (the class of fish, amphibian, mammal, etc.) tissue cells may appear many special anti-freeze proteins or cold adaptation-associated proteins for existence and developm...
关键词：RATS DURING cold adaptation BRAIN LYMPHOCYTE CELL-FREE endogenous protein synthesis system SDSPAGE autoradiograph. 
NUCLEAR RECONSTITUTION IN THE Xenopus laevis EGG EXTRACTS CELL-FREE SYSTEM
《Chinese Science Bulletin》1991年第6期508-513,共6页蔡树涛 张博 青婧 张传茂 翟中和 
This work was supported by grants from the National Natral Science Foundation of China and Doctoral Fondstion from the National Committee for Education.
Ⅰ. INTRODUCTIONNuclear reconstitution (nuclear assembly )was reported by several laboratories in recent years and regarded as an important advance in cell biology. In vitro nuclear assembly system provides a good mod...
关键词：nuclear RECONSTITUTION CELL-FREE system XENOPUS LAEVIS EGG extracts LAMBDA DNA. 
NITROGEN FIXATION BY CELL-FREE EXTRACT FROM Frankia PURE CULTURES
《Chinese Science Bulletin》1987年第9期646-647,共2页陈冠雄 禹阿东 吴杰 
Extracts with nitrogenase activity have been prepared from 20 different organism,since J. E. Carnahan et al. demonstrated nitrogeuase activity in the crude extract from Clostridium pasteurianum in 1960. The properties...
关键词：CLOSTRIDIUM CELL CRUDE summarized REMOVE 
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