云南高校图书馆联盟文献共享服务平台- MUTAGENESIS

MUTAGENESIS: 作品数：216被引量：439H指数：9; 导出分析报告; 相关领域：生物学医药卫生更多>>; 相关作者：袁高庆黄永禄林纬王静朱建华更多>>; 相关机构：湖南农业大学东南大学广西大学北京大学更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划中国博士后科学基金国家高技术研究发展计划更多>>

Developing superior alleles of yield genes in rice by artificial mutagenesis using the CRISPR/Cas9 system被引量：10: 《The Crop Journal》2018年第5期475-481,共7页Liyu Huang Ru Zhang Guangfu Huang Yanxia Li Getachew Melaku Shilai Zhang Haitao Chen Yanjuan Zhao Jing Zhang Yesheng Zhang Fengyi Hu; the Department of Sciences and Technology of Yunnan Province (2016BB001);the National Basic Research Program of China (2013CB835200);a Key Grant of Yunnan Provincial Science and Technology Department (2013GA004); Rice yield is an important and complex agronomic trait controlled by multiple genes.In recent decades,dozens of yield-associated genes in rice have been cloned,many of which can increase production in the form of loss...; 关键词：RICE CRISPR/Cas9 Gn1a DEP1 Gene EDITING

Genome editing in Drosophila melanogaster： from basic genome engineering to the multipurpose CRISPR-Cas9 system被引量：5: 《Science China(Life Sciences)》2017年第5期476-489,共14页Xingjie Ren Kristof Holsteens Haiyi Li Jin Sun Yifan Zhang Lu-Ping Liu Qingfei Liu Jian-Quan Ni; supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China (2015BAI09B03, 2016YFE0113700);the National Natural Science Foundation of China (31371496, 31571320);the National Basic Research Program (2013CB35102); Nowadays, genome editing tools are indispensable for studying gene function in order to increase our knowledge of biochemical processes and disease mechanisms. The extensive availability of mutagenesis and transgenesi...; 关键词：genome editing Drosophila melanogaster MUTAGENESIS TRANSGENESIS CRISPR-Cas9

An in vitro recombination-based reverse genetic system for rapid mutagenesis of structural genes of the Japanese encephalitis virus: 《Virologica Sinica》2015年第5期354-362,共9页Ruikun Du Manli Wang Zhihong Hu Hualin Wang Fei Deng; supported by grants from the National Science Foundation of China (NSFC) (No.31125003 and No.31321001);the National Basic Research Program (973 Program) of China (Grants 2010CB530103); Japanese encephalitis virus(JEV) is one of the most common pathogens of severe viral encephalitis, which is a severe threat to human health. Despite instability of the JEV genome in bacteria, many strategies have been...; 关键词：JAPANESE ENCEPHALITIS virus(JEV) REVERSE genetic s

Effects of Mutagenesis by UV Irradiation and ^(60)Co-γ Irradiation on Fermentation of Xylose to Ethanol by Pichia stipitis: 《Agricultural Science & Technology》2015年第3期426-430,446,共6页刘阳曾璐熊冬梅熊兴耀苏小军蔡柳; 基金项目国家973计划课题(2012CB723004);湖南农业大学青年科学基金(12QN11)~~; ln this study, effects of UV irradiation and 60Co-γ irradiation on fermenta-tion of xylose to ethanol by Pichia stipitis were analyzed to investigate the optimal mutagenic conditions. According to the growth curve an...; 关键词：Pichia stipitis MUTAGENESIS XYLOSE FERMENTATION ETHANOL

A novel transposable Mu-like prophage in Bacillus alcalophilus CGMCC 1.3604(ATCC 27647)被引量：1: 《Virologica Sinica》2015年第1期63-65,共3页Junjie Yang Yimeng Kong Xuan Li Sheng Yang; supported by the National Basic Research Program(973 Program)of China(2014CB745100);the National Key Technologies R&D Program of China(2012AA022101); Dear Editor,Transposable phages,which are reproduced by transposition(Harshey,2012;Taylor,1963),have been widely applied in the field of biotechnology to manipulate operon/gene fusions,in vivo cloning,randomion mutage...; 关键词：cloning biotechnology reproduced MUTAGENESIS PHAGE sequencing DNA http Sanger CHROMOSOME

Mutagenesis at a-423^(Ile) of MoFe protein reduces the catalytic activity of nitrogenase in Klebsiella oxytoca: 《Chinese Science Bulletin》2014年第9期849-856,共8页Qingjuan Guo Tao Peng Tianju Chang Gang Zhang Wei Jiang Ying Li Jilun Li; supported by the National Basic Research Program of China(2010CB126504);the Chinese Universities Scientific Fund(KYCX2011023);State Key Laboratories for Agrobiotechnology(2012SKLAB01-2); Nitrogenase catalyzes the ATP-dependent reduction of dinitrogen to ammonia,the central process of biological nitrogen fixation.The mechanism of electron transfer from the P-cluster to the iron-molybdenum cofactor(FeMo...; 关键词：钼铁蛋白固氮酶催化活性产酸乙炔还原活性诱变 C2H2 双通道模式

An Efficient Rice Mutagenesis System Based on Suspension-Cultured Cells被引量：2: 《Journal of Integrative Plant Biology》2013年第2期122-130,共9页Yuan-Ling Chen Hui-Lin Liang Xing-Liang Ma Su-Lin Lou Yong-Yao Xie Zhen-Lan Liu Le-Tian Chen Yao-Guang Liu; supported by grants from the Ministry of Science and Technology of China(2012AA10A303);the Ministry of Agriculture of China(2011ZX08010-001); Plant mutants are important bio-resources for crop breeding and gene functional studies. Conventional methods for generating mutant libraries by mutagenesis of seeds with physical or chemical agents are of low efficie...; 关键词：RICE MUTAGENESIS EMS suspension cell culture mutant.

Effective Expression-Independent Gene Trapping and Mutagenesis Mediated by Sleeping Beauty Transposon被引量：3: 《Journal of Genetics and Genomics》2012年第9期503-520,共18页Perry B.Hackett; supported by the National Natural Science Foundation of China(No.30871442) to Z.Cui;the National Basic Research Program of China(No.2012CB944500); Expression-independent gene or polyadenylation[poly（A）]trapping is a powerful tool for genome-wide mutagenesis regardless of whether a targeted gene is expressed.Although a number of poly（A）-trap vectors have been...; 关键词：Poly（A） trapping Sleeping Beauty transposon Insertional mutagenesis HeLa cells Zebrafish embryos

Mutagenesis and selective breeding of a high producing ε-poly-L-lysine strain被引量：3: 《Frontiers of Chemical Science and Engineering》2012年第2期179-183,共5页Tian WANG Shiru JIA Zhilei TAN Yujie DAI Shuai SONG Guoliang WANG; ε-Poly-L-lysine （ε-PL） is an L-lysine linear homopolymer, which is produced by bacteria belonging to the Streptomycetaceae family and by ergot fungi. How- ever, the production of ε-PL by the wild bacteria strain ...; 关键词：e-poly-L-lysine PLASMA AHV Streptomyces diastatochromogenes FERMENTATION

Characterization and function analysis of a Halo-alkaline-adaptable Trk K^+ uptake system in Alkalimonas amylolytica strain N10被引量：2: 《Science China(Life Sciences)》2009年第10期949-957,共9页GUO YongHao1, XUE YanFen1, LIU Jun2, WANG QuanHui1 & MA YanHe1 1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China 2 Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York 10029, USA; Supported by the National Key Basic Research and Development Program of China (Grant Nos. 2007CB714301 and 2007CB707801);the National High Technology Research and Development Program of China (Grant No. 2007AA021306);the National Natural Science Foundation of China (Grant No. 30621005); By functional complementation of Escherichia coli mutants defective in potassium (K+) uptake, two genes that are required for K+ uptake in halo-alkaliphilic Alkalimonas amylolytica strain N10 were cloned. These two ge...; 关键词：K+ uptake system haloalkaliphile TrkH and TRKA alkaline-adaptable site directed MUTAGENESIS

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