PCC6803

作品数:14被引量:43H指数:4
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相关领域:生物学更多>>
相关作者:米华玲叶济宇邓勇沈允钢茹炳根更多>>
相关机构:中国科学院上海生命科学研究院北京大学中国科学院东南大学更多>>
相关期刊:《Science Bulletin》《生物工程学报》《Journal of Traditional Chinese Medical Sciences》《科学通报》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划中国博士后科学基金更多>>
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Synechocystis sp.strain PCC 6803定量PCR模板制备方法的改进被引量:2
《华中农业大学学报》2016年第6期44-51,共8页阳桂丹 张巨源 陈雯莉 
国家自然科学基金项目(31570048);中央高校基本科研业务费专项(2014PY003)
以Synechocystis sp.strain PCC 6803为实验菌株,设计改良制备定量PCR模板的方法;同时,对Synechocystis sp.strain PCC 6803总RNA的Trizol抽提法进行优化。结果显示,利用改良方法抽提得到的RNA结构更完整;同时,利用改良方法制备的模板...
关键词:定量PCR Synechocystissp.strain PCC6803 RNA抽提 qPCR模板制备 
Construction and application of the Synechocystis sp.PCC6803-ftnA in microbial contamination control in a coupled cultivation and wastewater treatment
《Journal of Environmental Sciences》2016年第8期174-181,共8页Yalei Zhang Chunmin Zhang Xuefei Zhou Zheng Shen Fangchao Zhao Jianfu Zhao 
supported by the National Key Technologies R&D Program of China(No.2012BAJ25B02)
Inspired by iron fertilization experiments in HNLC(high-nitrate, low-chlorophyll) sea areas,we proposed the use of iron-rich engineered microalgae for microbial contaminant control in iron-free culture media. Based ...
关键词:Synechocystis PCC6803-ftnA Municipal wastewater treatment Microbial contaminants 
Biotransformation of 6-deoxypseudoanisatin by Synechocystis sp. PCC6803
《Journal of Traditional Chinese Medical Sciences》2014年第2期135-139,共5页Zhi Wang Xiaodong Cui Chunmei Wang Jianmei Huang Di Geng 
Beijing University of Chinese Medicine(2013-JYBZZ-JS-139)Independent Project Topics Foundation.
Objective:To explore the ability of Synechocystis sp.PCC6803 in transforming 6-deoxypseudoanisatin.Methods:The experiment was performed by incubating 6-deoxypseudoanisatin with the freshwater cyanobacterium Synechocys...
关键词:Synechocystis sp.PCC6803 6-deoxypseudoanisatin Seco-prezizaane-type sesquiterpene lactone BIOTRANSFORMATION CYANOBACTERIUM 
Characterization of calcium deposition induced by Synechocystis sp. PCC6803 in BG11 culture medium被引量:7
《Chinese Journal of Oceanology and Limnology》2014年第3期503-510,共8页闫华晓 韩作振 赵辉 周仕学 迟乃杰 韩梅 寇小燕 张艳 徐琳琳 田晨晨 秦松 
Supported by the National Natural Science Foundation of China(Nos.40972043,41040018,41210104058,21176145,41372108,41302079);the Higher Educational Science and Technology Program of Shandong Province(No.J10LC15);the China Postdoctoral Science Foundation(No.2013M540560);the Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province,and SDUST Research Fund(No.2010KYTD103);the Open Project of Key Lab of Marine Bioactive Substance and Modern Analytical Technique,State Oceanic Administration,China(No.MBSMAT-2012-03);the Scientific and Technological Program of Qingdao(No.13-1-4-232-jch);the Domestic Visiting Scholar Program for Young Core Teachers in Shandong Universities,Shandong Province,China
Calcium carbonate (CaCO3) crystals in their preferred orientation were obtained in BG11 culture media inoculated with Synechocystis sp. PCC6803 (inoculated BG11). In this study, the features of calcium carbonate d...
关键词:Synechocystis sp. PCC6803 preferred orientation BIOMINERALIZATION calcium carbonate thermal stability 
Transcriptomic analysis of Synechocystis sp. PCC6803 under low-temperature stress
《Chinese Journal of Oceanology and Limnology》2014年第2期403-418,共16页刘志香 崔红利 刘正一 王寅初 崔玉琳 刘兆普 秦松 
Supported by the National Natural Science Foundation of China(No.40876082);the International Innovation Partnership Program:Typical Environmental Process and Effects on Resources in Coastal Zone Area;the Public Science and Technology Research Funds Projects of the Ocean(Nos.200905021,201205027);the Outstanding Young Scholars Fellowship of Shandong Province(Molecular Phycology,No.JQ200914);the Natural Science Foundation of Shandong Province(No.ZR2012DQ015);the Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences(No.2011A090100040)
In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDN...
关键词:Synechocystis sp. PCC6803 CYANOBACTERIA cDNA microarray TRANSCRIPTOMICS low temperature stress 
Role of two phosphohexomutase genes in glycogen synthesis in Synechocystis sp. PCC6803
《Chinese Science Bulletin》2013年第36期4616-4621,共6页LIU Lian HU HanHua GAO Hong XU XuDong 
supported by the National Natural Science Foundation of China(30825003)
Phosphohexomutases catalyze the interconversion between hexose-6-phosphate and hexose-1-phosphate and play important roles in polysaccharide synthesis.In Synechocystis sp.PCC 6803,sll0726 is predicted to encode PGM(ph...
关键词:糖原合成 集胞藻 E基因 铂族金属 多糖合成 金属活性 调控启动子 突变体 
Effect of Cl^- on photosynthetic bicarbonate uptake in two cyanobacteria Microcystis aeruginosa and Synechocystis PCC6803被引量:8
《Chinese Science Bulletin》2009年第7期1197-1203,共7页CHEN Zhen CHENG HuiMin CHEN XiongWen 
Supported by National Natural Science Foundation of China (Grant No. 30770172);Science Foundation for Distinguished Young Scholars of Hubei Province (Grant No. 2007ABB004)
Photosynthetic inorganic carbon utilization was investigated in two cyanobacteria Microcystis aeruginosa and Synechocystis PCC6803 grown in standing culture. Photosynthetic rates for the two algae reached about 10 tim...
关键词:铜绿微囊藻 光合速率 高氯 碳酸氢盐 蓝藻 血管内凝血 光合作用 藻类生长 
转基因集胞藻PCC6803培养条件的优化被引量:1
《中国海洋大学学报(自然科学版)》2007年第S2期115-120,共6页张晓青 张学成 臧晓南 
国家高技术研究发展规划项目(819-04-10);农业转基因生物安全管理办公室批准项目(2005-T104)资助
通过研究培养基中硝酸盐、磷酸盐、碳酸盐等单因子对转牙鲆生长激素基因的集胞藻PCC6803生长的影响,再对以上3营养因子进行三水平的正交实验,得到培养基中硝酸盐、磷酸盐、碳酸氢盐的优化组合。还对培养温度、光照强度、pH值进行三因素...
关键词:转基因集胞藻PCC6803 正交试验设计 优化 培养条件 
集胞藻PCC6803细菌光敏色素体外重组和光化学活性研究被引量:4
《生物工程学报》2004年第2期238-244,共7页董依然 冉勇 赵开弘 周明 
国家自然科学基金 (No .90 2 0 1 0 0 1和 30 2 70 32 6 )资助~~
采用聚合酶链式反应 (PCR)从集胞藻PCC680 3总DNA中扩增出细菌光敏色素全片段cph1及cph1(C 43 5) ,克隆于pBluescriptSK(+ ) ,然后插入表达载体pET3 0a进行高效表达。获得的脱辅基蛋白Cph1、Cph1(C 43 5)在合适的缓冲体系下分别与藻蓝胆...
关键词:集胞藻 PCC6803 细菌光敏色素 体外重组 光化学活性 DNA 克隆 表达 藻蓝胆素 PCB 
集胞蓝藻PCC6803含疏水亚基的NAD(P)H脱氢酶亚复合体的分离被引量:2
《生物化学与生物物理学报》2003年第8期723-727,共5页邓勇 叶济宇 米华玲 沈允钢 
国家重点基础研究发展规划项目 (973计划)(No .G19980 10 10 0);国家自然科学基金项目 (No.3 0 2 70 12 3)资助~~
利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化...
关键词:集胞蓝藻 NAD(P)H脱氢酶 疏水亚基 类囊体膜 
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