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Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior: 《Chinese Physics B》2024年第7期624-629,共6页蒋环杰王艳伟陈家媛胡丹潘海郭子龙陈虎; supported by the National Natural Science Foundation of China(Grant Nos.12174322 to HC and 12204124 to ZG);111 Project(Grant No.B16029);the Graduate Scientific Research Foundation of Wenzhou University(Grant No.3162023003034 to JH);research grant from Wenzhou Institute。; Single-molecule magnetic tweezers(MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dep...; 关键词：protein folding magnetic tweezers protein L

Advances in the study of protein folding and endoplasmic reticulum-associated degradation in mammal cells被引量：2: 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2024年第3期212-232,共21页Hong CAO Xuchang ZHOU Bowen XU Han HU Jianming GUO Yuwei MA Miao WANG Nan LI Jun ZOU; This work was supported by the National Natural Science Foundation of China(No.82071762);the Shanghai Key Lab of Human Performance(Shanghai University of Sport)(No.11DZ2261100);the 2021 Capacity Building of Shanghai Universities(No.21010503600),China。; The endoplasmic reticulum is a key site for protein production and quality control.More than one-third of proteins are synthesized and folded into the correct three-dimensional conformation in the endoplasmic reticulu...; 关键词：Endoplasmic reticulum-associated degradation(ERAD) Protein folding UBIQUITINATION Retrotranslocation

Structural insights into the functions of Raf1 and Bsd2 in hexadecameric Rubisco assembly被引量：1: 《Molecular Plant》2023年第12期1927-1936,共10页Ran Wang Hui Song Wenjuan Zhang Ning Wang Shijia Zhang Ruiqi Shao Cuimin Liu; the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDA28030102);the National Key Research and Developmental Program of China(2021YFF1000203-14,granted to W.Z.);the National Natural Science Foundation of China(32170260).; Hexadecameric formI Rubisco,which consisting consists of eight large(RbcL)and eight small(RbcS)subunits,is the most abundant enzyme on earth.Extensive efforts to engineer an improved Rubisco to speed up its catalytic ...; 关键词：RUBISCO CHAPERONE protein folding protein assembly assembly factor

Effect of chaperone–client interaction strength on Hsp70-mediated protein folding: 《Chinese Physics B》2023年第11期635-641,共7页邹禄军陆伽俊徐秀莲; Project supported by the National Natural Science Foundation of China (Grant Nos. 11305139 and 11974173);the HPC Center of Nanjing University。; Protein folding in crowding cellular environment often relies on the assistance of various chaperones. Hsp70 is one of the most ubiquitous chaperones in cells. Previous studies showed that the chaperone–client intera...; 关键词：protein folding molecular chaperone molecular dynamics HSP70

Protein Structure Prediction:Challenges,Advances,and the Shift of Research Paradigms被引量：2: 《Genomics, Proteomics & Bioinformatics》2023年第5期913-925,共13页Bin Huang Lupeng Kong Chao Wang Fusong Ju Qi Zhang Jianwei Zhu Tiansu Gong Haicang Zhang Chungong Yu Wei-Mou Zheng Dongbo Bu; the National Key R&D Program of China(Grant No.2020YFA0907000)lthe National Natural Science Foundation of China(Grant Nos.32271297,62072435,31770775,and 31671369)for providing financial support for this study and publication charges.; Protein structure prediction is an interdisciplinary research topic that has attracted researchers from multiple fields,including biochemistry,medicine,physics,mathematics,and computer science.These researchers adopt ...; 关键词：Protein folding Protein structure prediction Deep learning TRANSFORMER Language model

HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis: 《Plant Communications》2023年第3期244-257,共14页Silvina Mangano Alfonso Muñoz Lourdes Fernández-Calvino M.Mar Castellano; supported by the project RTI2018-095946-B-I00 and PID2021-126956OB-I00 from MICIU;by"Severo Ochoa Programme for Centres of Excellence in R&D"from the Agencia Estatal de Investigación of Spain(grants SEV-2016-0672 and CEX2020-000999-S to the CBGP);In the frame of this latter program,S.M.was supported with postdoctoral contracts.We also acknowledge the grant"Recualificación del profesorado universitario"from the Ministerio de Universidades to A.M.(UCO).; Gibberellins(GAs)play important roles in multiple developmental processes and in plant response to the environment.Within the GA pathway,a central regulatory step relies on GA-dependent degradation of the DELLA transc...; 关键词：HSP70-HSP90 organizing protein seed germination thermomorphogenesis RGA protein folding protein stability

Protein folding mechanism revealed by single-molecule force spectroscopy experiments被引量：1: 《Biophysics Reports》2021年第5期399-412,共14页Hao Sun Zilong Guo Haiyan Hong Ping Yu Zhenyong Xue Hu Chen; This research was supported by the National Natural Science Foundation of China(11874309 and 11474237);111 project(B16029);the Start-up grant of Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2021008).; Force spectroscopy experiments use mechanical force as a control factor to regulate the folding and unfolding process of proteins.Atomic force microscopy has been widely used to study the mechanical stability of prote...; 关键词：Protein folding Free energy landscape Force spectroscopy Molten globule state Transition state

Folding nucleus and unfolding dynamics of protein 2GB1: 《Chinese Physics B》2021年第2期116-120,共5页Xuefeng Wei Yanting Wang; Project supported by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDA17010504);the National Natural Science Foundation of China(Grant No.11947302)。; The folding of many small proteins is kinetically a two-state process with one major free-energy barrier to overcome,which can be roughly regarded as the inverse process of unfolding.In this work,we first use a Gaussi...; 关键词：protein folding 2GB1 two-state model folding nucleus

Application of topological soliton in modeling protein folding: Recent progress and perspective被引量：1: 《Chinese Physics B》2020年第10期24-32,共9页Xu-Biao Peng Jiao-Jiao Liu Jin Dai Antti J Niemi Jian-Feng He; Proteins are important biological molecules whose structures are closely related to their specific functions. Understanding how the protein folds under physical principles, known as the protein folding problem, is one...; 关键词：protein folding coarse-grained method Landau free energy function topological soliton

Different potential of mean force of two-state protein GB1 and downhill protein gpW revealed by molecular dynamics simulation: 《Chinese Physics B》2020年第7期99-103,共5页Xiaofeng Zhang Zilong Guo Ping Yu Qiushi Li Xin Zhou Hu Chen; Project supported by the National Natural Science Foundation of China(Grant Nos.11874309,11474237,and 11574310);the 111 Project,China(Grant No.B16029).; Two-state folding and down-hill folding are two kinds of protein folding dynamics for small single domain proteins.Here we apply molecular dynamics(MD)simulation to the two-state protein GB1 and down-hill folding prot...; 关键词：protein folding molecular dynamics simulation umbrella sampling potential of mean force

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