SMRT

作品数:78被引量:342H指数:10
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相关机构:内蒙古农业大学学研究院哈尔滨广厦学院清华大学更多>>
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湿地松体细胞胚胎发生过程的全长转录组测序及生物信息学分析
《林业与环境科学》2024年第5期10-20,共11页薛蕾 曾明 王哲 郭文冰 伍彩云 郭业皇 刘阳 
广东省基础与应用基础研究基金项目(2021A1515012442)。
为提高湿地松Pinus elliottii体细胞胚胎发生效率,探究湿地松体胚发生的分子机理,利用SMRT(Single molecule real-time)第三代测序技术对湿地松体胚发生过程进行了全长转录组测序。测序总共生成了77.99 Gb原始数据,经多步筛选、矫正及...
关键词:湿地松 体细胞胚胎发生 SMRT三代测序 全长转录组 
Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota
《Food Science and Human Wellness》2024年第5期2690-2702,共13页Qing Liu Teng Ma Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang 
supported by the National Natural Science Foundation of China (31720103911);the China Agriculture Research System of MOF and MARA;the Science and Technology Major Projects of Inner Mongolia Autonomous Region (2021ZD0014)
Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria.Thus,oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis.We recruited 9 car...
关键词:Postbiotics Oral microbiota Salivary microbiota Dental caries Pacific Biosciences(PacBio)single molecule real-time(SMRT)sequencing Dental filling 
Brightness temperature simulation of subarctic lake-ice evolution by HIGHTSI and SMRT model被引量:1
《Advances in Polar Science》2024年第3期303-323,共21页ZHANG Yixiao QIU Yubao PENG Chongtai Bin CHENG SHI Lijuan Matti LEPPARANTA 
supported by the National Science and Technology Major Project(Grant no.2022ZD0117202);the National Natural Science Foundation of China(Grant no.42101389);CAS President's International Fellowship Initiative(Grant no.2021VTA0007).
Microwave brightness temperature(TB)can be used to retrieve lake ice thickness in the Arctic and subarctic regions.However,the accuracy of the retrieval is affected by the physical properties of lake ice.To improve th...
关键词:lake ice evolution HIGHTSI SMRT ice thickness passive microwave brightness temperature 
重庆地区RhD变异型无偿献血者的基因多态性及其表型研究被引量:1
《中国输血杂志》2024年第8期879-885,共7页刘静怡 崔丹荔 王芳 黎美君 刘东 谢晓艳 陈敏 付威义 杨冬燕 张巧琳 
重庆市科卫联合医学科研项目(2023QNXM042);重庆市九龙坡区科技计划项目(2022-02-018-Y);重庆市卫生健康委医学科研项目(2024WSJK033)。
目的对重庆地区22例RhD变异型无偿献血者标本进行Rh血型血清学检测和三代基因测序,了解重庆地区RhD变异型的表型分布及其基因分型。方法选择2023年1—8月本中心参与无偿献血的人群作为研究对象。使用传统血清学方法对其进行RhD表型鉴定...
关键词:RhD变异型 RHD基因 SMRT 三代测序技术 血清学 重庆 
基于SMRT测序技术的塔里木马鹿冬季肠道菌群多样性分析被引量:1
《野生动物学报》2024年第1期58-66,共9页祁程 姜李欢 阿卜杜萨拉木·努尔麦麦提 王开彦 单文娟 钟林强 
新疆维吾尔自治区自然科学基金项目(2022D01C657);新疆大学科研启动项目(620321053);新疆大学自治区级大学生创新训练计划项目(S202210755079)。
为探究塔里木河流域塔里木马鹿(Cervus elaphus yarkandensis)冬季肠道菌群群落结构、多样性与功能,采用PacBio平台的单分子实时测序技术(single molecule realtime sequencing,SMRT)对塔里木河上游湿地自然保护区采集并筛选的25份塔里...
关键词:塔里木马鹿 肠道菌群 单分子实时测序(SMRT) PICRUSt功能预测 
SMRT-AgRenSeq-d in potato (Solanum tuberosum) as a method to identify candidates for the nematode resistance Gpa5被引量:2
《Horticulture Research》2023年第11期271-279,共9页Yuhan Wang Lynn H.Brown Thomas M.Adams Yuk Woon Cheung Jie Li Vanessa Young Drummond T.Todd Miles R.Armstrong Konrad Neugebauer Amanpreet Kaur Brian Harrower Stan Oome Xiaodan Wang Micha Bayer Ingo Hein 
supported by the Rural&Environment Science&Analytical Services(RESAS)Division of the Scottish Government through project JHI-B1-1,the Biotechnology and Biological Sciences Research Council(BBSRC)through award BB/S015663/1 and the Royal Society through award NAF\R1\201061;YW was supported through the CSC scholarship program,China.LB was supported through the East of Scotland Bioscience Doctoral Training Partnership(EASTBIO DTP);funded by the BBSRC award BB/T00875X/1.AK was supported through a Research Leaders 2025 fellowship funded by European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no.754380.
Potato is the thirdmost important food crop in theworld.Diverse pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the fa...
关键词:resistance LINKAGE CROPS 
传统清香型白酒发酵过程中细菌群落演替及自组装机理被引量:2
《食品科学》2023年第22期139-148,共10页乔美灵 任宇婷 张桂莲 杨玉珍 刘梦宇 孙子羽 陈忠军 满都拉 
国家自然科学基金地区科学基金项目(32060533);内蒙古农业大学食品科学与工程学院科技项目(SPKJ201912);内蒙古自治区自然科学基金面上项目(2020MS03008)。
基于PacBio SMRT测序技术对传统清香型白酒发酵过程中细菌物种组成和群落结构开展研究,进一步利用iCAMP模型探究发酵过程中细菌群落自组装机制。结果表明,在发酵过程中细菌多样性随发酵过程呈现先降低后升高趋势,其中乳杆菌属(Lactobaci...
关键词:清香型 酒醅 PacBio SMRT 群落演替 群落自组装 
Comprehensive analysis of the full-length transcripts and alternative splicing involved in clubroot resistance in Chinese cabbage
《Journal of Integrative Agriculture》2023年第11期3284-3295,共12页SU He-nan YUAN Yu-xiang YANG Shuang-juan WEI Xiao-chun ZHAO Yan-yan WANG Zhi-yong QIN Liu-yue YANG Zhi-yuan NIU Liu-jing LI Lin ZHANG Xiao-wei 
supported by the National Natural Science Foundation of China (31872945 and 31801874);the earmarked fund for China Agricultural Research System (CARS-23-G15);the Funds for Distinguished Young Scientists from Henan Academy of Agricultural Sciences, China (2021JQ03);the Innovation Team of Henan Academy of Agricultural Sciences, China (2021TD06)。
Chinese cabbage is an economically important Brassica vegetable worldwide, and clubroot, which is caused by the soilborne protist plant pathogen Plasmodiophora brassicae is regarded as a destructive disease to Brassic...
关键词:Chinese cabbage CLUBROOT full-length transcriptome SMRT sequencing alternative splicing 
SMRT sequencing and ddPCR reveal the complexity of developmental trajectories and temporal dynamics of gut bifidobacterial communities in infants
《Food Science and Human Wellness》2023年第5期1743-1750,共8页Xu Gao Tao Zhang Xiaoye Bai Qiannan Wen Dongyu Li Lai-Yu Kwok Heping Zhang Zhihong Sun 
supported by the National Natural Science Foundation of China (31972083);the Natural Science Foundation of Inner Mongolia Autonomous Region (2020ZD12);the Inner Mongolia Science and Technology Major Projects (2021ZD0014);the Inner Mongolia Science & Technology Planning Project (2022YFSJ0017);China Agriculture Research System of MOF and MARA。
Infant intestinal microbiome is closely linked with health and risk of disease. Bifidobacterium are important components of the infant gut and are known to confer various health effects on the host. However, few studi...
关键词:INFANTS Gut microbiota BIFIDOBACTERIUM Diversity Single-molecule real-time(SMRT)sequencing Droplet digital polymerase chain reaction 
不同地区人初乳中细菌多样性研究
《微生物学报》2023年第8期3173-3186,共14页张蕾 赵志鑫 赵飞燕 孙志宏 
内蒙古自治区科技计划(2022YFSJ0017)。
初乳中的微生物在婴儿生长发育过程中具有多种有益作用。【目的】本研究分析了影响初乳微生物组成的多个因素,为后续探讨人初乳菌群的相关研究提供新思路。【方法】通过PacBio SMRT测序技术对37份采集自湖北恩施地区人初乳样本中细菌16S...
关键词:初乳 地区 16S rRNA基因 PacBio SMRT测序 细菌多样性 
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