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检索条件："关键词=RNA-seq "
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多形微眼藻在不同氮源条件下固碳途径的转录组解析及与其他微藻的比对被引量：1
《海洋与湖沼》2020年第5期1127-1135,共9页王甫文 李迎 甄毓 刘乾 
国家重点研发计划,2017YFC1404402号;国家自然科学基金,41976133号;青岛海洋科学与技术国家实验室鳌山科技创新计划项目,2016ASKJ02号。
本研究通过转录组技术对不同氮源培养下的多形微眼藻(Minutocellus polymorphus)固碳途径进行分析。通过对转录本进行NR、NT、Swiss-Prot和KOG富集分类及GO、KEGG相关代谢途径和基因差异表达分析,构建了多形微眼藻固碳途径。研究发现该...
关键词：多形微眼藻 固碳 转录组 
斜纹夜蛾四龄幼虫暴食相关基因的鉴定和通路分析被引量：3
《植物保护学报》2021年第6期1281-1290,共10页张赞 彭莉舒 李坤 钱坤 相栋 何林 
国家自然科学基金(32001962);中央高校基本科研业务费专项资金(SWU118094);博士后出站留(来)渝资助基金(2018LY43);西藏自治区重点研发计划(XZ202101ZY0006N)。
为绿色高效防治斜纹夜蛾Spodoptera litura,采用高通量测序技术对斜纹夜蛾暴食前期(3龄期)和暴食初始期(4龄期)进行转录组测序,筛选暴食前期和暴食初始期斜纹夜蛾差异基因,并对其高表达基因的代谢通路进行KEGG富集分析,随机选择缬氨酸...
关键词：斜纹夜蛾 幼虫 暴食期 转录组测序 代谢通路 实时荧光定量PCR 
Overview of available methods for diverse RNA-Seq data analyses被引量：16
《Science China(Life Sciences)》2011年第12期1121-1128,共8页CHEN Geng WANG Charles SHI TieLiu 
supported by the National Basic Research Program of China (Grant Nos. 2010CB945401, 2007CB108800);National Natural Science Foundation of China (Grant Nos. 30870575,31071162,31000590);Science and Technology Commission of Shanghai Municipality (Grant No. 11DZ2260300)
RNA-Seq technology is becoming widely used in various transcriptomics studies;however,analyzing and interpreting the RNA-Seq data face serious challenges.With the development of high-throughput sequencing technologies...
关键词：next generation sequencing TRANSCRIPTOME RNA-Seq data analysis TRANSCRIPTOMICS 
Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways
《Environment & Health》2024年第5期301-310,共10页Yifen Guo Yingao Yang Zixuan Zhou Changyuan Zhao Yanchun Li Huanying Zhou Shuyue Ren Yan Gu Zhixian Gao 
supported by Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety,Tianjin Institute of Environmental and Operational Medicine.
Parabens(PBs),especially propylparaben,commonly used in consumer products,pose environmental and health concerns.This study explored propylparaben’s cytotoxicity on HTR-8/SVneo human trophoblast cells,revealing signi...
关键词：PROPYLPARABEN HTR-8/SVneo cells TROPHOBLAST APOPTOSIS RNA-seq 
De novo transcriptome assembly of RNA-Seq reads with different strategies被引量：4
《Science China(Life Sciences)》2011年第12期1129-1133,共5页CHEN Geng YIN KangPing WANG Charles SHI TieLiu 
supported by the National Basic Research Program of China (Grant Nos. 2010CB945401, 2007CB108800);National Natural Science Foundation of China (Grant Nos. 30870575, 31071162,31000590);the Science and Technology Commission of Shanghai Municipality (Grant No. 11DZ2260300)
De novo transcriptome assembly is an important approach in RNA-Seq data analysis and it can help us to reconstruct the transcriptome and investigate gene expression profiles without reference genome sequences.We carri...
关键词：RNA-SEQ de novo transcriptome assembly next generation sequencing 
Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24被引量：5
《Journal of Integrative Plant Biology》2013年第7期631-642,共12页Zhenzhen Xu Chaojun Zhang Xueyan Zhang Chuanliang Liu Zhixia Wu Zuoren Yang Kehai Zhou Xiaojie Yang Fuguang Li 
supported by the National Science Fund for Distinguished Young Scholars(31125020);the Innovation Scientists and Technicians Troop Construction Projects of Henan Province
To get a broader view on the molecular mechanisms underlying somatic embryogenesis （SE） in cotton （Gossypium hirsutum L.）, global analysis of cotton transcriptome dynamics during SE in different sister lines was p...
关键词：AUXIN COTTON CYTOKININ RNA-Seq somatic embryogenesis. 
Finding neoepitopes in mouse models of personalized cancer immunotherapy
《Frontiers in Biology》2016年第5期366-375,共10页Sahar Al Seesi Alok Das Mohapatra Arpita Pawashe Ion I. Mandoiu Fei Duan 
BACKGROUND： Cancer immunotherapy uses one＇s own immune system to fight cancerous cells. As immune system is hard- wired to distinguish self and non-self, cancer immunotherapy is predicted to target cancerous cells s...
关键词：cancer immunotherapy tumor antigens neoantigens neoepitopes differential agretopic index （DAI） RNA-SEQ single nucleotide variant （SNV） 
Evaluation of heat stress effects on cellular and transcriptional adaptation of bovine granulosa cells
《Journal of Animal Science and Biotechnology》2020年第2期348-366,共19页Adnan Khan Jinhuan Dou Yachun Wang Xiaolong Jiang Muhammad Zahoor Khan Hanpeng Luo Tahir Usman Huabin Zhu 
support by Modern Agro-industry Technology Research System(CARS-36);the Program for Changjiang Scholar and Innovation Research Team in University(IRT_15R62).
Background:Heat stress is known to affect follicular dynamics,oocyte maturation,and fertilization by impairing steroidogenic ability and viability of bovine granulosa cell(bGCs).The present study explored the physiolo...
关键词：Bovine granulosa cells Differentially expressed genes FOLLICLES Heat stress RNA-SEQ 
基于RNA-seq技术对不同品种猪背最长肌差异表达基因的筛选与注释被引量：9
《西北农林科技大学学报（自然科学版）》2016年第6期1-8,共8页李小金 钱坤 刘林清 王重龙 周杰 
安徽省生猪产业技术体系项目(AHCYTX-06-10);安徽省生猪现代农业项目(13000101);安徽省农业科学院科技创新团队建设项目(13C0405)
【目的】筛选皖南花猪和大约克猪背最长肌组织差异表达基因。【方法】采集皖南花猪和大约克猪背最长肌(各3头),测定其肉质性状指标,并提取其RNA,采用高通量转录组测序(RNA-seq)技术进行测序,对所获序列进行GO和Pathway显著性富集分析。...
关键词：RNA-SEQ 皖南花猪 大约克猪 差异表达基因 背最长肌 
基于RNA-seq的两种柳树转录组微卫星特征比较分析被引量：2
《分子植物育种》2019年第5期1558-1566,共9页郑纪伟 周洁 王保松 何旭东 
江苏省农业科技自主创新资金(CX(14)2024);江苏省"六大人才高峰"项目(2016-NY-036);江苏省"333高层次人才培养工程"项目(BRA2017518)共同资助
随着高通量测序技术的飞速发展,转录组测序为EST-SSR标记的开发提供了重要的信息资源。为阐明柳树中EST-SSRs的变化情况,本研究以垂柳和簸箕柳转录组序列为来源,分析并比较了两种柳树中EST-SSR的分布规律及特征。结果表明:垂柳与簸箕柳...
关键词：RNA-SEQ 微卫星 EST-SSR 柳树 
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