热带假丝酵母利用酒糟水解液发酵生产木糖醇的初步研究  

Xylitol production with fermentation of lees hydrolysate by Candida tropicalis

作　　者：王铮[1] 陈红英[1] 沈才洪[1] 樊林[1] 胡承 

机构地区：[1]四川大学生物资源与生态环境教育部重点实验室,四川成都610064 [2]泸州老窖集团食品与发酵工程研究所,成都610064

出　　处：《中国酿造》2006年第3期44-47,共4页China Brewing

摘　　要：对热带假丝酵母(C.tropicalis)AY91009利用酒糟(丢糟)水解液发酵木糖醇进行了初步研究。结果表明:最佳发酵时间48h,最佳种子龄22h。摇瓶分批发酵工艺条件的最佳组合是:起始pH5.5,接种量15%(v/v),装液量135mL,氮源加入量为10mL含有10g/L酵母膏和20g/L蛋白胨的有机氮源。除水解液本身含有的木糖外,1/10的葡萄糖加入量(w/w)有利于菌体生长和木糖醇的转化,蔗糖则会抑制木糖醇的生成。培养基中添加6g/L的NaCl、3g/L的KH2PO4、0.2g/L的MgSO4.7H2O有利于木糖醇的积累。Xylitol production by fermentation of Candida tropicalis AY91009 using wine lees liquid was studied, The resuk showed that the optimal fermentation time was 48 h and inoculum time 22 h. The optimal shaking flask fermentation conditions were： initial pH 5.5, inoculttm 15% （v/v）, volume charge 135 ml in 250 ml flasks, and nitrogen source 10 ml mixed with 10 g/L yeast extract and 20 g/L tryptone. Besides the xylose contained in the lees hydrolysate, the 1/10 addition of glucose also promoted the conversion of xylitol yield and the growth of bacteria. Sucrose inhibited the production of xylitol. Addition of 6 g/L NaCl, 3 g/L KH2PO4, and 0.2 g/L MgSO4·7H2O to culture media enhanced xylitol accumulation.

关 键 词：酒糟水解液 热带假丝酵母 木糖醇 发酵 

分 类 号：TQ923[轻工技术与工程—发酵工程]