食源假单胞菌16S rRNA鉴定及其食品腐败特性的研究  

Identification of 16S rRNA and research on food spoilage traits of Pseudomonas spp.isolated from food

作　　者：綦国红[1] 董明盛[1] 陈晓红[1] 李除夕[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品工业科技》2006年第11期49-51,共3页Science and Technology of Food Industry

基　　金：国家(863)高技术研究发展计划(2002AA248041)江苏高技术研究发展计划(BG2004322)

摘　　要：从市售鱼中分离的三株革兰氏阴性菌FML05-1、FML05-2、FML05-3,经16s rRNA鉴定为假单胞菌属。该菌是一种导致食品腐败的重要腐败细菌。选取代表菌株的16S rRNA序列,利用phylip软件网上构建系统进化树,同时对其蛋白水解活性、嗜铁素产生和生物膜形成等与食品腐败相关的特性进行检测,发现FML05-2具有很强的蛋白水解活性和生物膜的形成能力,并且在脱脂牛奶中生长还产生大量的嗜铁素,菌株FML05-1具有很强的生物膜形成能力,但蛋白水解活性较弱,在LB及脱脂牛奶中生长均不产生嗜铁素,而菌株FML05-3不具有上述三种特性。Three Gram-negative bacteria were isolated from commercial fish and considered by analysis of 16S rRNA sequence to belong to Pseudomonas group, an important group of microorganisms that cause food spoilage. Phylogenetic relationships were constructed with the Phylip software by web interface. The characteristics （Protase ,siderophore （S）, formation biofilm） involved in food spoilage were assayed. The results showed that the FML05-2 was capable of the forming biofilm, proteolytic activity and producing siderophore （S） in skim milk, FML05-1 showed the capacity of biofilm formation and only very weak proteolytic activity, and was unable to produce siderophores in skim milk and LB. But FML05-3 did not show the traits as the above.

关 键 词：假单胞菌 16S RRNA 食品腐败 

分 类 号：TS205[轻工技术与工程—食品科学]