粗壮脉纹孢菌所产纤维素酶的性质研究  

Study on the Properties of Cellulase in Neurospora crassa

作　　者：余玮[1] 邓泽元[1] 范亚苇[1] 余永红[1] 吴丹[1] 

机构地区：[1]南昌大学食品科学与工程系,食品科学教育部重点实验室,江西南昌330047

出　　处：《食品科学》2006年第12期50-53,共4页Food Science

基　　金：教育部长江学者和创新团队发展计划资助项目(IRT0540);江西省教育厅课题资助项目(200626)

摘　　要：本文对粗壮脉纹孢菌(Neurosporacrassa)所产纤维素酶的一般酶学性质进行研究,得出的结论如下:该酶的最适催化反应pH为4.8～5.3,最适催化反应温度为45～50℃,在50℃及pH为4.8时该酶稳定。CMC、C1和β-葡萄糖苷酶活力变化范围有一定差别。锰离子对该酶活力有一定的增强作用,而铜离子和铝离子对其活力有明显的抑制作用。在产酶培养基中加入洗衣粉可以促进产纤维素酶活力,而洗洁精则会抑制产酶活力。The general properties of cellulase in Neurospora crassa was studied in this paper. The results were as tbilows： the optimal conditions of enzymatic hydrolysis were at pH4.8～5,3, 45～50℃, Cellulase was stable at pH4.8 and 50℃, end the activity of CMCase, Clase and β-glucosidase were different from one another. The cellulase was activated by Mn^2＋, but inhibited by Cu^2＋ and Al^3＋. Adding washing powderinto producting enzyme substrate could improve the activity of cellulase; but detergent liquid could inhibit the activity.

关 键 词：粗壮脉纹孢菌(Neurospora crassa) 纤维素酶 酶性质 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]