利用浓醪酒糟培养单细胞蛋白的研究  

Study on Single Cell Protein Production from Distiller's Grains with Candida tropicalis

作　　者：丁重阳[1] 吴天祥[2] 张梁[1] 石贵阳[1] 章克昌[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室 [2]贵州大学化学工程学院,贵州贵阳550003

出　　处：《酿酒科技》2007年第12期95-98,共4页Liquor-Making Science & Technology

基　　金：国家科技部"十五"攻关项目资助(2001BA501A01)

摘　　要：利用玉米原料酒精浓醪发酵产生的酒糟,对其培养热带假丝酵母生产SCP进行了初步的研究。经过优化后的发酵条件为:酒糟中添加0.2‰CaCl2和1.0‰KH2PO4,起始pH为4.5,装液量为30mL/500mL三角瓶;发酵26h,获得的最高菌浓为11.3亿/mL;发酵过程中酵母基本消耗完酒糟中的糖,同时还消耗了酒糟中的有机酸。发酵得到的SCP样品中含水分9.47%,粗蛋白47.73%,真蛋白45.09%,取得了较好的效果。Single Cell Protein （SCP） was produced from distiller＇s grains, which came from high gravity ethanol fermentation of corn, by using Candida tropicalis in shake-flasks. The optimum conditions of fermentation were as follows： CaCl2 0.2 %c, KH2PO4 1.0 ‰, initial pH value as 4.5,and the cultivation performed in 500 mL shake flask containing 30 mL medium. Under the above conditions, 1,130 million/mL C. tropicalis was obtained. HPLC analysis indicated that C. tropicalls consumed almost all the sugar, and organic acids were also consumed. In result, satisfactory results were achieved and the produced SCP samples contained 9.47 % water, 47.73 % crude protein and 45.09 % true protein.

关 键 词：热带假丝酵母 单细胞蛋白 酒糟 酒精浓醪发酵 

分 类 号：TS261[轻工技术与工程—发酵工程]