罗非鱼鱼皮多肽的超滤分离及其抗氧化活性研究  

Study on Separation of Peptides Derived from Oreochromis niloticus Skin with Ultra-filtration Technology and Its Antioxidative Activity

作　　者：刘成梅[1] 梁汉萦[1] 刘伟[1] 万婕[1] 

机构地区：[1]南昌大学食品科学教育部重点实验室,中德食品工程中心,江西南昌330047

出　　处：《食品科学》2008年第5期227-230,共4页Food Science

基　　金：教育部长江学者和创新团队发展计划项目(IRT0540);国家创新基金项目(05C26213600936);国家农业科技成果转化资金项目(2006GB2C500148)

摘　　要：采用超滤技术分离罗非鱼鱼皮蛋白酶解液(TSP-I),研究超滤压力、时间和浓度对膜渗透通量、渗透增量和膜效能的影响。结果表明:超滤处理的压力为28Psi,时间为40min,浓度为5%的操作条件下,膜渗透通量为9.98LHM,渗透增量为2.68g/10min,膜效能为3.41g/m2·min,超滤处理达最佳效果。利用分光光度法检测了抗坏血酸和超滤后所得多肽粉(TSP-II)对超氧阴离子自由基(O2·)、羟基自由基(·OH)、二苯代苦味酰自由基(DPPH·)及双氧水(H2O2)的清除能力。实验发现:超滤处理所得多肽具有一定的抗氧化能力,TSP-II对四种自由基的清除作用顺序为:H2O2>·OH>O2·>DPPH·。以清除H2O2的能力为代表相比较,0.80mg/mlTSP-II和0.03mg/ml抗坏血酸清除H2O2的能力相当。Peptides derived from TSP-Ⅰ was separated by ultra-filtration technology, the effects of pressure,temperature and concentration of peptides on ultra-filtration permeate flux, permeate increment and membrane efficiency. The results showed that the optimum conditions are： 5% TSP-Ⅰ, pressure 28 Psi and time 40 min. Under the condition, ultra-filtration permeate flux is 9. 98 LHM, ultra-filtration permeate increment is 2.68 g/10min and membrane efficiency is 3.41 g/m2·min. The capacities on scavenging O2·, ·OH, DPPH· and H2O2 of VC and TSP-Ⅱ were investigated by spectrophotometry. Study informed that TSP-Ⅱ had a certain extent antioxidation, the order of scavenging ability is H2O2 〉 · OH 〉 02 · 〉 DPPH · On scavenging H2O2, 0.80 mg/ml TSP-II is equivalent to 0.03 mg/ml VC.

关 键 词：罗非鱼鱼皮 胶原多肽 抗氧化作用 分光光度法 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]