无溶剂体系中酶催化合成结构脂质条件初探  

Enzymatic synthesis of structured lipids in a solvent-free system

作　　者：陈翔[1,2] 王瑛瑶[1] 栾霞[1] 张蕊[1] 赵玉生[2] 

机构地区：[1]国家粮食局科学研究院,北京100037 [2]郑州轻工业学院食品工程系,郑州450002

出　　处：《中国油脂》2010年第3期35-38,共4页China Oils and Fats

基　　金：公益性科研院所基金项目

摘　　要：以菜籽油和辛酸为原料,在无溶剂体系中用脂肪酶催化酸解合成结构脂质。对6种不同来源的脂肪酶进行筛选,结果表明Lipozyme RMIM催化活性高、Sn-1,3位特异性强。以Lipozyme RMIM为催化用酶,考察了反应时间、反应温度、底物比(菜籽油与辛酸摩尔比)、加酶量、体系水分含量对酸解反应的影响。结果表明,在反应时间15 h,反应温度50℃,底物比1∶4,加酶量10%条件下,辛酸合成率达40%。Lipase-catalyzed acidolysis of rapeseed oil with caprylic acid was performed to produce low-calorie structured lipids in a solvent-free system.Six commercial lipases were screened for their ability to catalyze acidolysis and the results indicated that the immobilized Lipozyme RM IM had more catalysis ability and Sn-1,3-specificity.Acidolysis parameters,such as time,reaction temperature,substrate molar ratio,enzyme load,water content were investigated.Under these conditions of 10% of the total weight of substrates for immobilized lipase,molar ratio of rapeseed oil to caprylic acid 1∶4 and reaction 15 h at 50℃,the incorporation of caprylic acid into rapeseed oil reached 40%.

关 键 词：菜籽油 辛酸 脂肪酶 酸解 结构脂质 

分 类 号：TQ645.6[化学工程—精细化工]