培养基组成对雅致放射毛霉发酵生产壳聚糖的影响  

Effects of the Fermentative Culture Medium on Production of Chitosan from Actinomucor elegans

作　　者：刘珍利[1] 赵华[1] 楚宁[1] 张洋[1] 

机构地区：[1]工业微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2010年第2期13-17,共5页Journal of Tianjin University of Science & Technology

基　　金：国家科技基础条件平台资助项目(2005DKA21204–10)

摘　　要：研究了发酵培养基中不同碳源、氮源、无机盐对雅致放射毛霉(Actinomucor elegans)合成壳聚糖的影响.采用单因素和响应面法优化了发酵培养基中玉米粉和玉米浆这两种主要成分的最佳添加量组合.结果表明,最佳培养基组成为(g/L):玉米浆68,玉米粉液化液42,KH2PO4 2,MgSO4 2.采用优化后的培养基,壳聚糖产量最大达到2.223,g/L,比优化前提高了64.30%.The effects of carbon source, nitrogen source and inorganic salts on production of chitosan from Actinomucor elegans were investigated. The proportion of two main components （corn steep liquor and corn flour）was optimized by single-factor design and response surface methodology. The results show that the optimal proportion （g/L） ： corn steep liquor 68, liquefied corn flour 42, KH2PO4 2, MgSO4 2. Under the above conditions, the yield of chitosan reached 2.223 g/L, which increased about 64.30% compared with non-optimization.

关 键 词：壳聚糖 雅致放射毛霉 玉米浆 玉米粉 响应面法 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]