鸡腿菇菌柄多糖提取工艺的研究  

Study on extraction technology of polysaccharide from stalk of Coprinus comatus

作　　者：李波[1] 索晓敏[1,2] 芦菲[1] 张建广[1] 葛静静[1] 李斌[2] 

机构地区：[1]河南科技学院食品学院,河南新乡453003 [2]华中农业大学食品学院,湖北武汉430070

出　　处：《食品工业科技》2010年第5期238-240,244,共4页Science and Technology of Food Industry

基　　金：河南省高校新世纪优秀人才支持计划(2006HANCET-17);河南科技学院重点科研项目资助基金

摘　　要：将鸡腿菇子实体的菌柄与菌盖分离后,分别进行干燥,得鸡腿菇菌柄干粉。本文以鸡腿菇菌柄粉为材料,对菌柄水溶性多糖和碱溶性多糖的提取工艺条件进行了研究。通过单因素实验和响应面中心组合实验设计,确定了菌柄水溶性多糖的最佳提取工艺条件:料液比1∶40、温度81℃、时间2.5h、提取2次,水溶性多糖得率为12.65%,多糖含量为70.50%。通过单因素实验确定了菌柄碱溶性多糖的最佳提取工艺条件:料液比1∶10,4%NaOH溶液,80℃提取2h,提取2次,碱溶性多糖得率为20.14%,多糖含量为38.51%。Stalk and cap of Coprinus comatus were separated,then stalk powder was obtained after drying respectively. The extraction technology of water-soluble polysaccharide（WSP）and alkali-soluble polysaccharide（ASP）of stalk powder were studied. According to the single-factor experiment and central composite experimental design principles by response surface analysis,the optimum extraction parameters of ASP were as follows：the ratio of raw material to water 1∶40（w/v）,extracting at 81℃ for 2.5h,twice. The yield of ASP was 12.65%,content of polysaccharide was 70.50%. Through single-factor experiment,the optimum extraction parameters of ASP were：NaOH solid/solvent ratio 1∶10,extracting with 4% NaOH at 80℃ for 2h,twice. The yield of WSP was 20.14%,content of polysaccharide was 38.51%.

关 键 词：鸡腿菇 水溶性多糖 碱溶性多糖 提取 菌柄 

分 类 号：TS201.3[轻工技术与工程—食品科学]