UV处理筛选耐高浓度乙醇酿酒酵母菌株  

Screen of ethanol tolerance strains Saccharomyces cerevisiae mutated by ultraviolet radiation

作　　者：杨东升[1] 朱丽元[1] 谢晓红[2] 罗先群[1] 

机构地区：[1]海南大学热带生物资源教育部重点实验室,海南海口570228 [2]海南省人民医院,海南海口570311

出　　处：《中国酿造》2010年第10期35-38,共4页China Brewing

基　　金：海南省教育厅科研基金资助项目(Hj2009-22)

摘　　要：通过对酒精酵母进行紫外诱变及2,3,5-氯化三苯基四氮唑(TTC)的鉴别培养,获得了10株呈红色菌落表型的突变株。诱变条件为:紫外(灯管功率20W)波长254nm,照射距离20cm,照射时间10s、60s和80s,获得正突变菌株Z8,可以耐受12%vol酒精。同时表明了酒精高产和酒精耐性有一定的相关性。Ten mutant strains of Saccharomyces cerevisiae showing red colony phenotype on the triphenyltetrazolium chloride medium were obtained in this paper. The mutation condition of ultraviolet lights were as followed： light strength 20 W, vertical distance 20 cm, wave length 254 nm and irradiation time 10 s, 60 s and 80 s, respectively. The strain Z8 tolerating 12%vol ethanol was obtained. The results also indicated that ethanol production was positively related with ethanol tolerance.

关 键 词：紫外诱变 酿酒酵母 酒精耐性 发酵 

分 类 号：TS261.1[轻工技术与工程—发酵工程]