乌鸡低聚肽体外抗氧化活性研究  

Antioxidant Activities in vitro of Black-bone Silky Fowl Oligopeptides

作　　者：刘文颖[1] 马永庆[1] 金振涛[1] 陈亮[1] 易维学[1] 蔡木易[1] 

机构地区：[1]中国食品发酵工业研究院

出　　处：《食品与发酵工业》2010年第10期19-23,共5页Food and Fermentation Industries

摘　　要：在对乌鸡低聚肽的基础理化成分、分子量分布和氨基酸组成进行分析的基础上,从DPPH自由基、羟基自由基和超氧阴离子自由基清除能力以及还原能力4个方面对乌鸡低聚肽的体外抗氧化活性进行了全面的考察。结果显示:乌鸡低聚肽分子量大多为1000u以下(96.74%),含量最高的是140～500u的小肽(78.53%);乌鸡低聚肽具有较强的体外抗氧化能力,抗氧化效果与质量浓度呈正相关,对DPPH自由基、羟基自由基和超氧阴离子自由基的半抑制浓度(IC50值)分别为4.5、4.5和12mg/mL,浓度为9.19mg/mL时的还原能力与0.02mg/mL的抗坏血酸相当。On the basis of compositions, molecular weight distribution and amino acid composition of black-bone silky fowl （BSF） oligopeptides, the antioxidant activities in vitro of BSF oligopeptides were generally evaluated from four respects by determination of the scavenging activities against DPPH radical, hydroxyl radical and superoxide radical as well as reducing power. Results indicated that the major molecular weight of BSF oligopeptides was less than 1 000 u （96. 74% ）, and small peptides with the molecular weight range from 140 to 500 u were dominated （78.53%）. BSF oligopeptides had strong antioxidant ability in vitro and the antioxidation correlated positively with mass concentration. The median inhibitory concentrations （ICs0 values） of DPPH radical, hydroxyl radical and superoxide radical scavenging activities were 4.5, 4.5 and 12 mg/mL, respectively. Meanwhile, the reducing power of BSF oligopeptides at 9.19mg/mL correlated with that of ascorbic acid at 0.02mg/mL.

关 键 词：乌鸡 低聚肽 氨基酸组成 分子量分布 抗氧化活性 

分 类 号：S831[农业科学—畜牧学]