白腐真菌产木聚糖酶发酵条件的优化及酶学性质研究  

Study on the optimization of xylanase fermentation conditions by white rot fungi and the enzymological properties

作　　者：岳晓禹[1,2] 张恒业[1] 刘艳娟[3] 牛天贵[2] 刘相东[4] 

机构地区：[1]郑州牧业工程高等专科学校质检系,河南郑州450011 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]河南商业高等专科学校,河南郑州450044 [4]中国农业大学工学院,北京100083

出　　处：《食品工业科技》2011年第4期197-200,共4页Science and Technology of Food Industry

基　　金：郑州牧专博士科研启动资金资助项目

摘　　要：通过正交实验对白腐真菌Pleurotus ostreatus SYJ042产酶条件进行了优化,研究了该酶的酶学性质。结果表明:最适发酵条件为:碳源为5%的麸皮和玉米芯(1:1),氮源为0.5%的蛋白胨,接种量为每50mL发酵液接4块菌丝块,发酵时间为8.5d。该酶的最适温度是50℃,最适pH为5.4;Zn2+、Ca2+对酶活性影响较小;Ag+、MnO2-4影响较大。By the orthogonal test, the optimal fermented condition of xylanase secretion by white rot fungi was obtained：2.5% wheat bran ＋ 2.5% corn cob, 0.5% peptone,4 pieces per 50mL liquid volume, 8.5d. By the experimental method in the thesis,some properties of the xylanase were obtained ：the optimal temperature was at 50℃.The optimal pH was 5.4.Ag ＋ and MnO4^2- evidently impacted on the xylanase activity.

关 键 词：木聚糖酶 发酵条件 酶学性质 

分 类 号：TS201.3[轻工技术与工程—食品科学]