黑曲霉B0201固态浅盘生料发酵产单宁酶工艺研究  

Tannase production by uncooked material and solid-state fermentation of Aspergillus niger B0201 with shallow trays

作　　者：李广神[1,2] 邱树毅[1,2] 吴鑫颖[2] 谢晓莉[1,3] 王维维[1,3] 

机构地区：[1]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [2]贵州大学化学与化工学院,贵州贵阳550003 [3]贵州大学生命科学学院,贵州贵阳550025

出　　处：《中国酿造》2011年第4期98-101,共4页China Brewing

基　　金：贵州省科技厅工业攻关项目(NO.GY20093022);贵州省自然科学基金项目(NO.GY20082091)

摘　　要：通过单因素实验,对黑曲霉B0201固态生料浅盘发酵产胞外单宁酶进行了研究。结果表明:最佳发酵工艺为接种龄为30h,接种量为20%,五倍子含量为30%,固液比为1.6:1,培养温度为0h^48h、35℃,48h^96h、25℃,96h^120h、30℃,装量为100g,培养期间每隔24h搅拌一下,单位酶活达21.08U。Optimal productions of tannase by Aspergillus niger B0201 using uncooked material and solid-state fermentation with shallow trays were studied through single factor experiments in this paper.The results showed that the optimal fermentation conditions were as follows： seed age 30 h,inoculum 20%,gallnut content 30%,the ratio of solid to liquid 1.61,temperature 35℃（0 h^48 h）,25℃（48 h^96 h） and 30℃（96 h^120 h）,medium content 100 g and stirring every 24h during fermentation.Under these conditions,the enzyme activity reached 21.08 U.

关 键 词：黑曲霉 单宁酶 酶活 固态生料浅盘发酵 

分 类 号：TQ925[轻工技术与工程—发酵工程]