利用法夫酵母JMU-MVP14联产发酵虾青素和功能性低聚糖的研究  

Simultaneously Production of Astaxanthin and Functional Oligosaccharide by Phaffia Rhodozyma JMU-MVP14 Strain

作　　者：邵曼[1] 倪辉[1,2,3] 蔡慧农[1,2,3] 肖安风[1,2] 洪清林[1] 苏文金[1,2,3] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]福建省教育厅食品微生物与酶工程技术研究中心,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021

出　　处：《中国食品学报》2011年第7期4-13,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(20702019);福建省产学研重大专项项目(2010N5009)

摘　　要：为了提高法夫酵母JMU-MVP14的利用价值,探索在高产发酵虾青素的基础上联产发酵虾青素和功能性低聚糖。用高效液相色谱及液-质联用技术对法夫酵母JMU-MVP14发酵液中低聚糖成分进行鉴定,考察糖的种类、蔗糖的添加量、取样时间、发酵温度及pH对法夫酵母JMU-MVP14产低聚糖的影响。用7L罐研究联产发酵虾青素和低聚糖的工艺可行性。结果表明,法夫酵母JMU-MVP14合成的低聚糖为新科斯糖和蔗果三糖。法夫酵母JMU-MVP14合成低聚糖的最佳条件是:蔗糖质量浓度80～110g/L,温度22～25℃,pH5.0～7.0;在7L罐中发酵至72h时补充蔗糖溶液至80g/L,发酵74～78h时新科斯糖和蔗果三糖的含量达到峰值,新科斯糖、蔗果三糖及总低聚糖含量分别达到10.22、2.82和13.04g/L。此时,虾青素的体积产率、细胞产率分别为42.45mg/L和5.84mg/g。本文提供了一种提高法夫酵母利用价值的途径,用法夫酵母JMU-MVP14可同时发酵虾青素和功能低聚糖。To promote the practical values of Phaffia rhodozyma JMU-MVP14 strain,which had special high yield of astaxanthin,the novel fermentation process for the coproduction astaxanthin and functional oligosaccharide was explored.The component of oligosaccharide in fermented broth of Phaffia rhodozyma JMU-MVP14 was identified by HPLC and HPLC-MS.Effects of carbohydrates composition,sucrose concentration,incubation time,fermentation temperature and pH on the yield of functional oligosaccharide,as well as the technological feasibility of coproduction astaxanthin and oligosaccharide in 7 L fermentor were investigated.The results showed that the oligosaccharides produced by Phaffia rhodozyma JMU-MVP14 were kestose and neokestose.The optimal fermentation condition for Phaffia rhodozyma JMUMVP14 producing oligosaccharide was sucrose concentration within 80～110 g/L,temperature within 22～25 ℃,pH within 5.0～7.0.As 80 g/L sucrose was added into the fermented broth which was cultured in the 7L fermentor for 72 h,Phaffia rhodozyma JMU-MVP14 produced highest yield of neokestose and kestose in 74 ～78 h,at where the concentration of neokestose,kestose and total oligosaccharide were 10.22,2.82 and 13.04 g/L respectively.Meanwhile,the volumetric yield and cell yield of astaxanthin were 42.45 mg/L and 5.84 mg/g respectively.This study provides a new approach of utilization of Phaffia rhodozyma which enables simultaneously fermentation of astaxanthin and functional oligosaccharides.

关 键 词：法夫酵母 高产菌株 虾青素 功能性低聚糖 联产 

分 类 号：TQ929[轻工技术与工程—发酵工程]