一株产酯化酶细菌的筛选  

Screening of A Esterifying Enzyme-producing Bacteria Strain

作　　者：张秀红[1,2] 刘秋林[2] 孔健[3] 赵景龙 

机构地区：[1]山西杏花村汾酒厂股份有限公司,山西汾阳032205 [2]山西师范大学生命科学学院,山西临汾041000 [3]山东大学生命科学学院,山东济南250100

出　　处：《酿酒科技》2012年第6期56-58,共3页Liquor-Making Science & Technology

摘　　要：从汾酒大曲(后火曲、红心曲、清茬曲)中筛选出1株产酯化酶较高的菌株,从菌落外形及显微镜观察初步确认是葡萄球菌。该菌株在液体发酵培养基中,以37℃恒温摇床培养48 h,发酵液酶活力可达25.00 U/mL;对该菌株的发酵培养基进行碳源和氮源优化。结果表明,以豆饼粉作氮源,玉米粉为碳源,于37℃、180 r/min下摇床培养48 h,酶活力可达到33.33 U/mL。A bacteria strain with strong capability of producing esterifying enzyme was screened out from Fenjiu Daqu,such strain was identified as Staphylococcus by its cell shape and colony characterization.When grown in liquid fermenting culture medium,37 ℃ for 48 h,its enzyme activity could reach up to 25.00 U/mL.After the optimization of carbon source and nitrogen source of the culture medium（soybean cake powder used as nitrogen source and corn flour as carbon source）,the enzyme activity of the strain could reach up to 33.33 U/mL（37 ℃,180 r/min for 48 h）.

关 键 词：微生物 细菌 酯化酶 酶活力 优化 汾酒大曲 

分 类 号：TS261.11[轻工技术与工程—发酵工程]