灰树花加硒米糠培养基液态发酵产富硒多糖的研究  

Study on liquid fermentation with Grifola frondosa in the medium contained partial rice bran for Se-polysaccharides

作　　者：赵莉[1] 刘伟民[1] 顾慧敏[1] 张建[1] 郭春梅[1] 李永转[1] 刘丽丽[1] 杨小明[1] 赵杰文[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品工业科技》2012年第18期199-201,204,共4页Science and Technology of Food Industry

基　　金：江苏省社会发展科技计划项目(BS2006037);江苏镇江市农业支撑科技项目(NY2008043);江苏高校优势学科建设工程(PAPD)资助项目(2011年);南京高源环保工程有限公司横向联合课题

摘　　要：为高值化利用稻谷加工副产品米糠,开发新型的保健食品,在加入米糠的培养基中,对灰树花液态发酵生产富硒灰树花多糖进行研究。通过单因素实验选择得到灰树花富硒液体培养过程中Se的最适添加量,并以菌丝干重、多糖含量、硒含量和富硒率为评价指标,按U12(124)均匀设计的实验方案,探索富集硒的多糖的产物与葡萄糖、米糠添加量和添加方式之间的关系。实验结果为:在硒含量为0～300mg/L下液态深层培养,菌丝均能生长,硒最适添加量为10mg/L,但超过50mg/L,菌丝生长和多糖生产都受到明显地抑制。回归得到方程,并通过方程预测优化培养基:硒、米糠、葡糖糖的添加量分别为0.11、7.5、2.5g/100mL时,此时菌丝干重为1.32g/100mL,菌丝多糖为0.444g/100mL。Using rice bran efficiently to prepare the Se-polysaccharides health foods on liquid fermentation,the bio-sorption of selenium on liquid fermentation by the Grifola frondosa was tested in the medium with rice bran.The optimal Se-adding was selected as single-factor and U12（124） uniform design experiments were made.Results showed that when Se-adding was 10mg/L,but once it exceeded 50mg/L,the mycelium growth and enrichment of polysaccharides were obviously inhibited.By calculating with SPSS,regression models were obtained.When the yield of Se in mycelium reached the maximum,the efficiency of mycelium and polysaccharides were 1.32g/100mL and 0.444g/100mL with Se-adding 0.11mg/100mL,rice bran 7.5g/100mL and glucose 2.5g/100mL.

关 键 词：灰树花 米糠 多糖 硒 回归模型 

分 类 号：TS201.1[轻工技术与工程—食品科学]