反相液相色谱同时测定糖品中的防腐剂和甜味剂  

Determination of Preservative and Sweetener in Sugar by Reverse-Phase HPLC

作　　者：陈红香[1] 王桂华[1] 李家威[1] 陆剑华[1] 陈嘉敏[1] 李海乔[1] 郭剑雄[1] 

机构地区：[1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室国家糖业质量监督检验中心,广东广州510316

出　　处：《甘蔗糖业》2012年第5期58-62,共5页Sugarcane and Canesugar

基　　金：广东省科技计划项目(2011B040300001)

摘　　要：本文建立了用反相液相色谱同时测定糖品中的苯甲酸、山梨酸、脱氢乙酸、安赛蜜、糖精钠等防腐剂和甜味剂的方法。方法采用Venusil XBP-C18(5μm,4.6×250mm)反相色谱柱,流动相为20mmol/L乙酸铵-甲醇,流速为1.0mL/min,柱温为30℃,进样量为10μL。该方法的测定低限为1.0 mg/L,线性范围为10～100mg/L,加标回收率为89.85%～103.60%,相对标准偏差为1.50%～3.26%(n=4)。结果表明,该方法简便、可靠,适合对糖品中防腐剂和甜味剂的同时检测。Reverse-phase HPLC was applied to the determination of benzoic acid, sorbic acid, dehydroacetic acid, acesulfame K, sodium saccharin in sugar. The analysis column was Venusil XBP - C18 （5 μm, 4.6×250 mm）, the mobile phase was Methanol - 20 mmol/L NHgAc, the flow rate was 1.0 mL/min. The column temperature was 30 ℃, and the injection volume was 10 μL. The limit of quantitation was 1.0 mg/L. The linear plots were obtained between 10 and 100 mg/L. Overall recoveries were between 89.85% and 103.60%, and RSD （n = 4） value were 1.50% and 3.26%. Results showed this method was simple and accurate, it could be applied for determination of preservative and sweetener in sugar.

关 键 词：反相液相色谱 防腐剂 甜味剂 糖品 

分 类 号：TS247[轻工技术与工程—制糖工程]