不同发酵条件对发酵桑叶茶生物活性成分含量的影响  

Effects of Different Fermentation Conditions on the Contents of Bioactive Ingredients in Fermented Mulberry Leaf Tea

作　　者：肖洪[1] 黄先智 沈以红 丁晓雯[1] 张亚琼[1] 梁菡峪[1] 张迪[1] 

机构地区：[1]西南大学食品科学学院,重庆市农产品加工重点实验室,重庆400715 [2]家蚕基因组生物学国家重点实验室,重庆400715

出　　处：《食品科学》2013年第23期216-220,共5页Food Science

基　　金：国家现代农业(蚕桑)产业技术体系建设专项(CARS-22-ZJ0503);重庆市科委应用技术项目(cstc2012gg-yyjs80022)

摘　　要：目的:研究不同发酵条件下发酵桑叶茶中脱氧野尻霉素(DNJ)等生物活性成分含量变化。方法:采用HPLC法测DNJ含量,比色法测定黄酮、多糖、多酚含量。结果:与对照组比较,不同温度发酵后发酵桑叶茶中的多糖、黄酮和多酚含量均降低,发酵温度为30℃时发酵桑叶茶中DNJ含量增加了7.37%,黄酮含量略微降低;不同发酵时间发酵后发酵桑叶茶中的DNJ含量增加,在发酵时间为6h时DNJ含量最高增加7.10%,多糖含量增加6.45%;黄酮含量降低,发酵4h时多酚含量增加20.00%;菌种接种量为4×107CFU时发酵桑叶茶中DNJ含量增加4.23%,多糖含量最高增加3.68%,而黄酮和多酚含量降低。结论:发酵温度30℃、发酵时间6h、菌种接种量4×107CFU,最有利于发酵桑叶茶的功能性质。Objective： To investigate the changes of 1-deoxynojirimycin （DNJ） and other bioactive compounds in fermented mulberry leaf tea with different fermentation conditions. Methods： DNJ was determined by HPLC. Flavonoids, polysaccharides and polyphenosl were determined by spectrophotometric methods. Results： Compared with those in mulberry leaves, the contents of flavonoids, polysaccharides and polyphenol in mulberry leaf tea fermented at any of the fermentation temperatures tested were decreased. DNJ content in mulberry leaf tea fermented at 30 ℃ was increased by 7.37%, whereas the content of flavonoids was slightly decreased. DNJ content in mulberry leaf tea fermented for different times was increased, with the highest increase by 7.10% at 6 h, accompanied by a 6.45% increase in polysaccharide content. The content of polyphenols was increased by 20.00% in mulberry leaf tea fermented for 4 h, while the amount of flavonoids became lower. The contents of DNJ and polysacchaddes were increased by 4.23% and 3.68%, respectively, but flavonoid and polyphenol contents were decreased in mulberry leaf tea fermented with an inoculums amount of 4 × 10^7CFU. Conclusion： Mulberry leaf tea fermented at 30 ℃, 6 h with 4 × 10^7CFU ofinoculum has the most favorable functional properties.

关 键 词：发酵 桑叶茶 脱氧野尻霉素 黄酮 多糖 多酚 

分 类 号：S886.9[农业科学—特种经济动物饲养]