不同冷冻方式及冻藏温度对海湾扇贝闭壳肌氨基酸组成的影响  

Effects of different freezing processes and frozen storage temperature on amino acids of argopecten irradiams muscle

作　　者：崔瑞颖[1] 张志胜[1] 郑乾魏[1] 冉丁[1] 梁帅峰 李娜[1] 

机构地区：[1]河北农业大学食品科技学院,保定071000

出　　处：《食品科技》2014年第3期97-100,共4页Food Science and Technology

基　　金：国家海洋局海洋公益性行业科研专项(201205031)

摘　　要：采用柱前衍生反相高效液相色谱法测定不同冷冻方式[(-25±1)℃冰箱冷冻、(-35±1)℃冰柜冷冻、(-80±1)℃超低温冷冻和液氮冷冻]及冻藏温度[(-25±1)℃、(-35±1)℃]对海湾扇贝闭壳肌氨基酸组成的影响。以2,4-二硝基氯苯作为柱前衍生剂,流动相为醋酸盐缓冲溶液和乙腈,梯度洗脱。采用C18柱分离,紫外检测器检测。研究结果表明:不同冷冻方式间,除(-25±1)℃冰箱冷冻和(-35±1)℃冰柜冷冻对样品间氨基酸组成影响差异不显著外(p>0.05),其他处理间样品差异均显著(p<0.05)。不同冻藏温度对样品氨基酸组成的影响差异不显著(p<0.05)。因此,海湾扇贝闭壳肌的冷冻综合考虑营养及成本,宜选择(-25±1)℃冰箱冷冻及(-25±1)℃冻藏。The effects of different freezing processes, such as （-25±1） % refrigerator freezing, （-35±1） % refrigerator freezing, （-80±1） % ultra-low temperature freezing and liquid nitrogen freezing, and different frozen storage temperature[（-25±1） ℃, （-35±1） ℃] on amino acids of Argopecten irradiams muscle were studied by pre-column derivatization high performance liquid chromatography. A derivation reagent of 2,4- dinitrochlorobenzen was used for sample derivatization. The conditions for the derivation and hydrolyzation of amino acids were optimized. Detection conditions were as follows： mobile phase-acetate buffer and acetonitrile in gradient elution, separation column C18 and UV detection. Results showed that： In different freezing processes, （-25±1） % refrigerator freezing and （-35±1） % refrigerator freezing had no significant difference on amino acid （p〉0.05）, other processing sample differences were significantly （p〈0.05）; The influence of different frozen storage temperature on amino acid differences was not si gnificant （p〉0.05）. Therefore, considering nutrition and costs of Argopecten irradias, （-25±1） ℃ refrigerator freezing and frozen storage at （-25±1） % were better on amino acids of Argopecten irradiams muscle.

关 键 词：冷冻方式 冻藏温度 海湾扇贝 氨基酸 高效液相色谱法 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]