顶空固相微萃取-气相色谱-质谱联用法分析酵母浸膏辐照挥发性成分  

Analysis of Volatile Compounds from Irradiated Yeast Extract by Headspace Solid Phase Micro-Extraction Coupled with Gas Chromatography-Mass Spectrometry

作　　者：耿胜荣[1] 夏和舟[1] 鉏晓艳[1] 陈玉霞[1] 叶丽秀[1] 熊光权[1] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,湖北武汉430064

出　　处：《食品科学》2014年第6期55-59,共5页Food Science

基　　金：国家自然科学基金青年科学基金项目(11105048);湖北省农业科技创新中心项目(2013-620-007-001)

摘　　要：采用顶空固相微萃取-气相色谱-质谱联用法分析酵母浸膏辐照后的挥发成分。结果表明:未经辐照的酵母浸膏萃取得到13种挥发性成分,其中含酸类1种、醛类2种、醇类2种、烯烃类1种、含氮化合物3种,主要成分为乙酸、吲哚、2-乙基己醇。辐照后的酵母浸膏萃取得到35种挥发性成分,其中酸类1种、醛类7种、醇类2种、烯烃类4种、酮类2种、含氮化合物14种、含硫化合物2种、酯类1种、苯类2种。挥发物的种类和含量均随辐照剂量的增加而增加。A method for determining volatile compounds from irradiated yeast extract using headspace solid phase microextraction （HS-SPME） and gas chromatography-mass spectrometry （GC-MS） was developed in this paper. The results showed that 13 volatile compounds were detected from unirradiated yeast extract including 1 acid, 2 aldehydes, 2 alcohols, 1 alkene and 3 nitrogen compounds and the major compounds were acetic acid, indole and 2-ethyl-l-hexanol. Totally 35 volatile compounds including 1 acid, 7 aldehydes, 2 alcohols, 4 alkenes, 2 ketones, 14 nitrogen compounds, 2 sulfur compounds, 1 ester and 2 benzenes were detected from irradiated yeast extract. The number and contents of volatile compounds increased with increasing irradiation dosage.

关 键 词：酵母浸膏 顶空固相微萃取 气相色谱-质谱联用仪 挥发性成分 辐照 

分 类 号：TS202[轻工技术与工程—食品科学] TL99[轻工技术与工程—食品科学与工程]