多重实时荧光定量PCR检测肴肉中的特定腐败菌  

Multiplex real-time quantitive PCR detection of specific spoilage organisms in Yao meat

作　　者：周慧[1] 支竹伟 刘涵[1] 付殊满 董英[1] 肖香[1] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013

出　　处：《粮食与食品工业》2014年第3期86-89,92,共5页Cereal & Food Industry

基　　金：江苏省高等学校大学生创新创业训练计划项目(项目编号:201310299018)

摘　　要：本试验以真空包装水晶肴肉中的3株特定腐败菌屎肠球菌(Enterococcus faecium)、中间耶尔森菌(Yersinia intermedia)和清酒乳杆菌(Lactobacillus sakei)为研究对象,通过克隆3株菌的特异性DNA片段,构建重组质粒作为阳性模板,建立一种多重实时荧光定量PCR方法同时检测这3株特定腐败菌,结果表明:建立的多重实时荧光定量PCR具有较高的特异性和灵敏度,同时还具有很好的稳定性,可用于真空包装水晶肴肉贮藏期间特定腐败菌数量的检测,从而可以间接评价产品的货架期。In this experiment, the multiplex real- time quantitive PCR method for detecting 3 strains of specific spoilage organisms （SSOs） in vacuum- packaged Yao meat - Enterococcus faeciurn, gersinia intermedia and Lactobacillus sakei was established by constructed recombinant plasmids as template. The results show that： the multiplex real - time quantitive PCR method has high specificity and sensitivity, also has good stability. Therefore, this method can be used to detect the number of SSOs in vacuum- packed Yao meat during storage, which can indirectly evaluate the shelf life of the product.

关 键 词：肴肉 实时荧光定量PCR 特定腐败菌 检测 

分 类 号：TS201.3[轻工技术与工程—食品科学]