一株产耐高温碱性脂肪酶菌株的筛选及产酶条件优化  

Screening of a thermostable alkaline lipase producing strain and optimization of fermentation condition for Lipase production

作　　者：杜萍萍[1] 张莹莹[1] 申培立 李志辉[1] 于宏伟[1] 卢海强[1] 苏旭东[1] 张伟[1] 檀建新[1] 

机构地区：[1]河北农业大学食品科技学院,河北省农产品加工工程技术中心,河北保定071001

出　　处：《食品工业科技》2015年第9期188-193,共6页Science and Technology of Food Industry

基　　金：多谷物主食冷冻面团的关键技术研究(13227105D);植物多酚类功能性物质对A形成的影响(冀人社字[2010]195号)

摘　　要：用Tween平板法从采集自河北省的122份土样中筛选到一株脂肪酶活性较高的菌株Lipa1318。综合其形态学和生理生化特征以及16S r DNA的序列比对结果,确定该菌株为粘质沙雷氏菌(Serratia marcescens)。其所产胞外脂肪酶的最适反应温度为60℃,最适p H为8.0,是一种耐热的碱性脂肪酶。摇瓶发酵实验证明该菌株的最适产酶条件为:葡萄糖1.0%,牛肉膏1.0%,Triton X-100 1.5%,橄榄油0.50%,Ca Cl20.50mmol/L,发酵液初始p H为8.0,30℃培养96h,通过发酵条件的优化使酶活提高了3.6倍。Using the plate assay method with Tween 80 as substrate of lipase,122 soil samples collected from Hebei province were screened and a bacterial strain Lipa1318 with high lipase activity was obtained. The results of morphological features,the physiological and biochemical characteristics and 16 S r DNA sequence alignment analysis demonstrated that the isolate was Serratia marcescens. The optimal temperature and pH of the extracellular lipase were 60℃ and 8.0,which indicated that the lipase was a thermostable alkaline lipase. The optimal fermentation condition for high lipase production in flask were as follows： glucose 1.0%,beef extract 1.0%,Triton X-100 1.5%,olive oil 0.50%,Ca Cl20.50 mmol / L,the initial pH（ 8.0） and temperature（ 30℃）,culture time（ 96h）.Under these conditions,the lipase activity was increased by 3.6 times.

关 键 词：脂肪酶 粘质沙雷氏菌 发酵条件 

分 类 号：TS201.3[轻工技术与工程—食品科学]