降解黄曲霉毒素B_1菌株的发酵条件优化及降解机制  

Optimization of Fermentation Condition for an AFB_1-Degrading Strain and Preliminary Exploration of Degradation Mechanism

作　　者：邵帅[1] 戴军[1] 杜馨[1] 王常高[1] 林建国[1] 蔡俊[1] 

机构地区：[1]湖北工业大学发酵工程教育部重点实验室工业发酵湖北省协同创新中心,湖北武汉430068

出　　处：《食品科学》2016年第5期138-143,共6页Food Science

基　　金：湖北省自然科学基金重点项目(2009CDA059)

摘　　要：目的:鉴定一株降解黄曲霉毒素B_1(aflatoxin B_1,AFB1)的霉菌HSK8,通过优化其发酵条件提高AFB_1降解率,并初步探索其降解机制。方法:通过形态学和内部转录间隔区(internal transcribed spacer,ITS)、18S r RNA和26S r RNA序列分析对该菌株进行鉴定,并通过单因素试验对发酵条件进行优化,采用薄层层析对AFB_1降解产物进行研究。结果:经鉴定该株霉菌为夏孢生枝孢(Cladosporium uredinicola)。其发酵条件经优化确定为:发酵温度28℃、装液量75 m L/250 m L、接种量15%、发酵时间36 h、初始p H 8.0。薄层层析观察到一个不同于AFB_1的荧光斑点。结论:发酵条件优化后,AFB_1降解率从40.68%提升到68.96%,提高了69.52%;AFB1降解产物能在365 nm波长处发出蓝色荧光。In this study, a mould isolated for degrading aflatoxin B1 was reported. Morphological studies coupled with internal transcribed spacer（ITS）, 18 S ribosomal RNA gene sequence, 26 S ribosomal RNA gene sequence analyses indicated that the mould was Cladosporium uredinicola. Through single-factor experiments, the fermentation conditions were determined as follows： temperature 28 ℃, medium loading volume 75 m L/250 m L, inoculumamount 15%（V/V）, fermentation time 36 h, initial p H 8.0. Under these conditions, the AFB1 degradation efficiency was 68.96%, a 69.52%increase over that before optimization（40.68%）. On the thin-layer chromatography（TLC） plate, a new dot was found which exhibited blue fluorescence at 365 nm.

关 键 词：黄曲霉毒素B1 鉴定 发酵条件 降解产物 

分 类 号：TS201.3[轻工技术与工程—食品科学]