液相色谱串联质谱法检测食品中4种人工合成着色剂  

Determination of 4 kinds of synthetic colorants in food by liquid chromatography-tandem mass spectrometry

作　　者：郑新华[1] 王乐[1] 韩焕美[1] 何桂华[1] 张爱霞[1] 陈晞[1] 

机构地区：[1]济南出入境检验检疫局,济南250014

出　　处：《食品安全质量检测学报》2016年第4期1412-1418,共7页Journal of Food Safety and Quality

基　　金：山东出入境检验检疫局科研项目(SK201424;SK201616)~~

摘　　要：目的建立固相萃取-液相色谱串联质谱法(SPE-LC-MS/MS)同时检测食品中新红、靛蓝、喹啉黄和酸性紫(49)4种人工合成着色剂的分析方法。方法样品经酸性水溶液提取,混合型弱阴离子固相萃取柱净化;采用Thermo C18色谱柱分离,以5 mmol/L乙酸铵溶液和甲醇为流动相梯度洗脱。质谱采用电喷雾离子源多反应监测模式(MRM)分析,外标法定量。结果 4种人工合成着色剂在0.2~1.0 mg/kg范围内呈线性相关,相关系数均大于0.99。方法的回收率介于70.0%~101.5%之间;相对标准偏差(RSD)(n=6)为2.3%~16.5%;检出限均为0.20mg/kg。结论该方法前处理简单,净化效果好,具有良好的灵敏度、回收率和重现性,适于食品中人工合成色素的检测。Objective To develop a method for the simultaneous analysis of New Red, Indigo Blue, Quinoline Yellow and Acid Violet 49 in food by solid phase extraction-liquid chromatography-tandem mass spectrometry（SPE-LC-MS/MS）. Methods The analytes were extracted from the samples by acidic water, then purified and enriched with a weak anion exchange SPE column. The 4 kinds of synthetic colorants were separated on a Thermo C18 column by gradient elution with 5 mmol/L ammonium acetate and methanol as the mobile phases. The analytes were determined by ESI-LC-MS/MS under multiple reaction monitoring（MRM） mode and quantified by external standard method. Results The results indicated that the calibration curve showed a good linearity of 4 kinds of synthetic colorants in the range of 0.2~1.0 mg/L（r^20.99）。The recoveries of 4 kinds of synthetic colorants were in the range of 70.0%~101.5% with RSDs（n=6） between 2.3% and 16.5%. The detection limits were all 0.20 mg/kg. Conclusion This method is suitable for the detection of synthetic colorants in food with simple preparation, good cleaning effect, high sensitivity and better repeatability.

关 键 词：液相色谱串联质谱法 固相萃取 食品 合成着色剂 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]