杜氏盐藻β-胡萝卜素超高压提取工艺优化  

Optimization of Extracting Technique Assisted by Ultrahigh Pressure Extraction of β-carotene from Dunaliella salina

作　　者：孙协军[1] 李秀霞[1] 冯彦博[1] 励建荣[1] 李颖畅[1] 吕艳芳[1] 刘雪飞[1] 

机构地区：[1]渤海大学食品科学与工程学院辽宁省食品安全重点实验室辽宁省高校重大科技平台"食品贮藏加工及质量安全控制工程技术研究中心",辽宁锦州121013

出　　处：《中国食品学报》2016年第3期88-94,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金："十二五"国家科技支撑计划(2012BAD29B06);辽宁省食品安全重点实验室开放课题(LNSA KF2011015)

摘　　要：对杜氏盐藻β-胡萝卜素超高压辅助提取工艺参数进行优化研究,结果表明,6种溶剂中,无水乙醇的提取效果最好。在超高压辅助提取条件下,各溶剂的β-胡萝卜素得率排序为无水乙醇>石油醚>95%乙醇>三氯甲烷>乙腈>乙酸乙酯;建立了超高压辅助提取杜氏盐藻β-胡萝卜素的数学模型,确定最佳工艺参数:液固比483m L/g,超高压压力346 MPa,保压时间13.7 min,结果β-胡萝卜素得率的预测值为1.07%。在优化条件下,β-胡萝卜素得率测定值为1.02%,说明提取模型的适应性良好。与常规的回流提取方法相比,超高压技术显著提高了杜氏盐藻β-胡萝卜素提取效率。Extracting technique assisted by ultrahigh pressure extraction method of β-carotene from D. Salina. was optimized. The result showed that absolute ethanol was the optimal solvent among 6 kinds of solvent selected, the mathematical model of EHV-assisted D. Salina β-carotene extraction was established, the optimal condition for extraction was that L/S 483 mL/g, ultrahigh pressure 346 MPa, retention time 13.7 min, under the optimal condition, the predicted β-carotene yield was 1.07%, and the measured value was 1.02%, it was described that the adaptable of model was good.Compared with conventional reflux extraction methods, extraction efficiency of β-carotene was improved significantly assisted by UHP method.

关 键 词：杜氏盐藻 Β-胡萝卜素 超高压提取 工艺 

分 类 号：TS202.3[轻工技术与工程—食品科学]