沙棘籽提取物抑菌能力的研究及其生物活性成分分析  

Research on the antimicrobial activity of the sea-buckthorn seed extract and analysis of its bioactive components

作　　者：扎格乐 边鑫[1] 赵莉[1] 徐敏[1] 王娜娜[1] 霍贵成[1] 

机构地区：[1]东北农业大学食品学院乳品科学教育部重点试验室,黑龙江哈尔滨150030

出　　处：《中国酿造》2016年第8期110-114,共5页China Brewing

基　　金：国家自然科学基金(31401512);国家高技术研究发展计划‘863计划’(2011AA100902)

摘　　要：选取蒙古国不同产地和不同培育方式的5种沙棘籽的活性成分进行了提取,研究了提取物对常见致病菌的抑菌能力,并对其中的生物活性成分进行了分析。结果表明,野生沙棘籽1号提取物对金黄色葡萄球菌的抑菌能力最强,抑菌圈直径可达(12.3±0.5)mm,而3号提取物具有广谱抑菌能力,对枯草芽孢杆菌、大肠杆菌和铜绿假单胞杆菌的抑菌能力最强,抑菌圈直径分别达到(11.5±0.5)mm、(12.3±0.2)mm和(11.9±0.3)mm,并且随着沙棘籽提取物的用量增加,其抑菌能力增强。沙棘籽提取物中主要的生物活性成分主要为植物甾醇、黄酮类物质、植物磷脂和维生素C,其中植物甾醇含量越高,对应的抑菌能力越强。The active constituents of five kinds of sea-buckthorn seed with different regions and different cultivation ways from Mongolia were extracted. The antimicrobial activity of extracts on common pathogenic bacteria was researched, and the bioactive components were analyzed. The results showed that the antimicrobial activity of wild sea-buckthom seed NO. 1 extract against Staphylococcus aureus was the strongest, and the diameter of inhibition zone was （12.3±0.5） ram. But wild sea-buckthom seed NO,3 extract had a broad-spectrum antibacterial ability, and the its antimicrobial activity on Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa was the strongest, the diameters of inhibition zone were （11.5±0.5） mm, （12.3±0.2） mm and （11.9±0.3） mm, respectively. With the increasing of sea-buckthom seed extract addition, its antimicrobial activity was stronger. The main bioactive components in sea-buckthorn seed extracts were phytosterol, flavonoids, plant phospholipids and vitamin C, and the higher phytosterols content was, the stronger antimicrobial activity was.

关 键 词：沙棘籽 生物活性成分 抑菌能力 

分 类 号：TS201.3[轻工技术与工程—食品科学]