纳米包装材料对金针菇木质化及相关酶活性的影响  

Effect of Nano-Packing on Lignification and Related Enzyme Activities of Flammulina velutipes

作　　者：方东路[1] 常诗洁 赵立艳[1] 安辛欣[1] 胡秋辉[2] 杨文建[2] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京财经大学食品科学与工程学院江苏省现代粮食流通与安全协同创新中心,江苏南京210023

出　　处：《食品科学》2016年第20期261-267,共7页Food Science

基　　金：江苏省自然科学基金项目(BK20141009);国家自然科学基金青年科学基金项目(31401552)

摘　　要：添加纳米银、纳米二氧化钛、凹凸棒土和纳米二氧化硅等材料制备一种纳米聚乙烯包装袋.跟踪检测4 ℃、相对湿度90%～95%贮藏过程中金针菇的菌柄最大剪切力、质量损失率、可溶性蛋白含量、木质素含量、苯丙氨酸解氨酶活性、肉桂醇脱氢酶活性、过氧化物酶活性和细胞微观结构的变化,分析纳米材料和普通聚乙烯材料包装对金针菇木质化劣变的影响.贮藏14 d后,纳米包装袋内金针菇菌柄最大剪切力、质量损失率和苯丙氨酸解氨酶、肉桂醇脱氢酶、过氧化物酶活性分别为1.191 kg、0.51%和20.72、41.23、10.09 U/mg,显著低于普通PE包装组（P〈0.05）;另一方面,纳米包装金针菇样品中的木质素含量相比最初仅增加4.7%,而普通PE包装组木质素含量则增加了14.2%.纳米包装袋能够有效抑制贮藏期间金针菇木质素的积累及相关酶活性的上升,很好地保持金针菇贮藏品质,延长贮藏时间.A polyethylene （PE） packing material containing nano-Ag, nano-TiO2, attapulgite and nano-SiO2 was preparedand applied in the preservation of Flammulina velutipes and its effects on sensory quality and lignification of F. velutipeswere determined during 14-day storage at 4 ℃. The influence of nano-packing material on the lignification of F. velutipes wasstudied by tracking the changes in texture, weight loss, soluble protein contents, lignin contents, the activities of phenylalnineammonialyase （PAL）, cinnamyl alcohol dehydrogenase （CAD）, and peroxidase （POD） as well as cell microstructure. Thenormal PE material was used as control. After 14 days of storage, the maximum shearing force, weight loss, PAL, CAD andPOD activities of F. velutipes packed with nano-packing material were 1.191 kg, 0.51%, 20.72 U/mg, 41.23 U/mg, 10.09 U/mg,respectively, which were significantly lower than those of the control （P 〈 0.05）. On the other hand, the lignin content innano-packaging samples increased by 4.7% while that in the control increased by 14.2% compared with the initial level.Nano-packing material has the potential to maintain the preservation quality and extend the shelf life of F. velutipes byinhibiting the accumulation of lignin and increasing the related enzyme activities.

关 键 词：金针菇 纳米材料 保鲜 木质化 货架期 

分 类 号：TS206.4[轻工技术与工程—食品科学]