刺参胶原蛋白的结构特征与聚集行为的耦合性研究  

Studies on the Interaction of Structure and Aggregation Behavior of Sea Cucumber Collagen

作　　者：彭喆[1] 侯虎[1] 樊燕[1] 司磊磊[1] 张凯[1] 李八方[1] Peng Zhe Hou Hu Fan Yan Si Leilei Zhang Kai Li Bafang(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandon)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国食品学报》2017年第3期28-37,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省自然科学基金项目(2014ZRE29099);中国博士后基金特别资助项目(2015T80751);国家自然科学基金项目(31401476);山东省重点研发计划项目(2016YYSP005)

摘　　要：采用X-射线衍射、傅里叶红外光谱、扫描电镜、流变学分析以及芘荧光探针等技术分析酶促溶性刺参胶原蛋白的结构特征、流变学特性及聚集特性,探讨不同浓度、pH和离子强度对其聚集特性的影响。结果表明:酶促溶刺参胶原蛋白呈完整的三螺旋结构,其聚集结构为疏松多孔状;在不同温度下,刺参胶原蛋白溶液的弹性模量G′和黏性模量G″呈剪切频率依赖性,而在浓度不同时则没有此现象,在低浓度时,大分子均不发生缠结和聚集;通过聚集实验发现随着质量浓度的增加,胶原蛋白会出现明显的聚集行为,当pH达7.6时易发生聚集,且Na Cl的加入使其聚集性发生滞后;随着聚集时间的增加,表征其聚集程度的芘荧光强度(Ⅰ_1、Ⅰ_3及Ⅰ_1/Ⅰ_3值)降低,说明聚集程度随着时间的延长而增加;其临界聚集质量浓度CAMC为0.813 g/L。试验表明,刺参胶原蛋白的聚集行为与其在溶液中的形态及氢键有关。In this study, the X-ray diffraction, FTIR, scanning electron microscope, rheologieal analysis and fluorescence probe were used to determine the structure, rheological properties and aggregate characteristics of enzymatic soluble sea cucumber collagen, while the effect of concentration, pH and ion strength was detected. The results showed that the collagen of sea cucumber kept the triple-helix structure, and it had a porous structure. It is found that the e- lastic modulus G＇ and the viscous modulus G＂ of sea cucumber collagen showed the dependence of shear frequency under different temperature, but it did not show the dependence when under different concentration, while when the concentration was lower, the aggregation would not occur. From the aggregation experiment, it is found that as the mass concentrations of collagen increasing, the aggregation behavior became obvious, and it was easy to aggregate when pH was 7.6, moreover, the NaC1 would delay the aggregation. During the aggregation, the I1, I3 values and I1/I3 ratio of pyrene decreased with the time prolonging, which indicated that the aggregation increased with time. The critical aggregation mass concentration （CAMC） of sea cucumber collagen was 0.813 g/L. This study shows that the aggregation behavior is related to the forms and hydrogen bond of sea cucumber collagen in solution.

关 键 词：刺参 胶原蛋白 结构 流变学特性 聚集特性 

分 类 号：TS201.21[轻工技术与工程—食品科学]