二次酶解法制备低能SLS型结构脂质工艺研究  

Studies on the Synthesis of Low-Energy SLS-Type Structure Lipid by Two Enzymatic Steps

作　　者：刘天一[1] 赵月[1] 王彤[1] 屈岩峰[1] 李婷婷[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2017年第6期75-83,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家十三五攻关课题(2016YFD0401402);国家自然科学基金面上项目(31571880)

摘　　要：以一级大豆油、乙醇及乙酸为原料,在脂肪酶Novozym 435催化作用下,先用一级大豆油与乙醇进行醇解工艺制得Sn-2-单甘脂,再将Sn-2-单甘脂与乙酸进行酶促酯化反应得到SLS型结构脂质,其优化的第2次酶解工艺条件是:反应时间11.6 h,加酶量8.0%,底物物质的量比5.5∶1,反应温度44.0℃。在此条件下乙酸插入率为64.5%,经分子蒸馏后获得纯度为94.3%,热值21.12 k J/g的SLS型结构脂质,与普通大豆油相比,热值降低了45%。Based on the level of soybean oil, ethanol, acetic acid as raw material, under the effect of lipase catalyzed Novozym 435, the first level of soybean oil and ethanol alcoholysis technology was made 2-monoacylglycerols;then 2-monoacylglycerols to enzymatic esterification reaction of acetic acid, end up with obtioned the SLS-type structure lipid, the technique of enzymolysis is improved. The results indicated that the optimum combination of the second enzymolysis, in the attendance of 8.0% lipase Novozym 435, reaction at 44.0 ℃and substrate molar ratio of 5.5∶1 for 11.6 h.Adopting the combination of the above the acetate acid incorporation was 64.5%, after the molecular distillation and purification of triglycerides for 94.3% purity and caloricity for 21.12 k J/g of SLS-type structure lipid, compared with ordinary soybean oil, the energy of obtained oils decreased 45%.

关 键 词：大豆油 酶解 结构脂质 脂肪酶 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程]