高温大曲中功能红曲霉菌株的筛选与功能浅析  

Screening and function analysis of functional Monascus strains in high-temperature Daqu

作　　者：张柱 黄钧[1] 周荣清[1,2] 雷梓伦 唐秋香 万营东 张宿义 秦辉 董异[2,3] 王超 王小军[2,3] 冉茂芳 ZHNAG Zhu;HUANG Jun;ZHOU Rongqing;LEI Zilun;TANG Qiuxiang;WAN Yingdong;ZHANG Suyi;QIN Hui;DONG Yi;WANG Chao;WANG Xiaojun;RAN Maofang(College of Light Industry Science and Engineering,Sichuan University,Chengdu 610056,China;National Solid State Brewing Engineering Technology Research Center,Luzhou 646699,China;Luzhou Laojiao Co.,Ltd.,Luzhou 646699,China)

机构地区：[1]四川大学轻工科学与工程学院,四川成都610056 [2]国家固态酿造工程技术研究中心,四川泸州646699 [3]泸州老窖股份有限公司,四川泸州646699

出　　处：《中国酿造》2024年第4期53-61,共9页China Brewing

摘　　要：该研究采用可培养技术从呈粉红色的高温大曲中分离红曲霉(Monascus)菌株,结合多元统计分析方法研究其在纯培养麦曲、模拟白酒固态发酵及强化大曲中的代谢特征,进而筛选优良的功能红曲霉菌株,并通过形态学观察和分子生物学技术对筛选菌株进行鉴定。结果表明,共分离筛选得到3株优良的功能红曲霉菌株,编号分别为H14、H26和H30。其中,菌株H14具有提高基酒产率(7.5%vol)、改善糟醅挥发性风味物质含量(33.12 mg/kg)和轮廓的潜力;菌株H30改善了高温大曲的酯化活性;菌株H26具有产特征风味化合物(吡嗪类物质)的潜力,其强化高温大曲中2,3,5,6-四甲基吡嗪的含量达0.27 mg/kg。经鉴定,菌株H14、H26和H30均为佛罗里达红曲霉(Monascus floridanus)。In this study,Monascus strains were screened from high-temperature Daqu with the pink color through the cultured technology.By combining multivariate statistical analysis methods,the metabolic characteristics of these strains in pure cultured wheat Qu,simulated Baijiu solid-state fermentation and fortified Daqu were studied.Subsequently,excellent functional Monascus strains were screened.The screened strains were identified by morphological observation and molecular biology techniques.The results showed that three excellent functional Monascus strains were isolated and screened,numbered as H14,H26 and H30.Among them,strain H14 showed potential ability to increase the base liquor yield(7.5%vol)and improving the profile of volatile compounds and their contents(33.12 mg/kg)in fermented grains.Strain H30 improved the esterification activity of high-temperature Daqu,and strain H26 demonstrated the potential to produce characteristic flavor compounds(pyrazines),with a content of 0.27 mg/kg for 2,3,5,6-tetramethylpyridine in high temperature fortified Daqu.Through identification,strains H14,H26,and H30 were all identified as Monascus floridanus.

关 键 词：高温大曲 功能红曲霉 筛选 鉴定 麦曲 酿酒 强化大曲 挥发性风味物质 

分 类 号：TS261.1[轻工技术与工程—发酵工程]