微生物谷氨酰胺转胺酶的分批发酵生产  

Production of Microbial Transglutaminase by Batch Fermentation

作　　者：刘新征[1] 王灼维[1] 杨秋明[2] 郭兴要[1] 韩兆鹏[1] 蔡慧农[2] 王璋[1,3] 

机构地区：[1]中国食品发酵工业研究院 [2]集美大学生物工程学院 [3]集美大学生物工程学院,厦门361201

出　　处：《食品科学》2003年第10期73-77,共5页Food Science

基　　金：国家"十五"科技攻关计划项目(2001BA708B03-05);科技部科研院所技术开发研究专项资金项目(NCSTE-2001-JKZX-006)

摘　　要：在首先采用我们自行设计的2L小型、便易的生化反应器,对我们研究室保藏的高产酶菌株链霉菌(Streptomyces sp.)WZFF.L-M168发酵生产微生物谷氨酰胺转胺酶(MTG)过程中发酵培养基的碳源、氮源和初始pH值的影响作用进行研究,确定培养条件后,逐级扩大发酵罐规模,在20L和200L发酵罐上以在线监控技术手段直接监测分析环境因素对MTG发酵生产的作用效果,确立各级发酵罐分批发酵的生产工艺条件。结果表明:碳氮源采用葡萄糖、淀粉和多价胨,初始pH值为7.0,接种量5%～10%,发酵过程中在线控制培养温度、pH、通气量和搅拌速度等各项参数指标分别为30±0.5℃、6.6～6.9,0.8～1.1vvm和200～400r/min较为适宜。在这优化技术条件下,200L发酵罐可以稳定生产酶活在2.75U/ml以上的MTG。After using a simple but efficient mini-bioreactor (2L) we equipped to study the effects of carbon source, nitrogensource and initial pH of fermentation broth on the production of microbial transglutaminase (MTG) by a new potent strain,Streptomyces sp. WZFF.L-M168 isolated from soil and preserved in our laboratories. The incubation conditions were established.The production was scaled up to 20 L and 200 L jar-fermenters through the utilization of on-line control techniques, and then thetechniques for batch fermentation of MTG were set up. The experimental results showed that the optimal processing param-eters achieved were 1.5% glucose and 1.0% soluble starch for the carbon source, 2.0% multi-peptone for the nitrogen source,initial pH 7.0, inoculum ratios of 5%-10%, that the following conditions were suitable during the MTG production with on-linecontrolled supervision: temperature 30℃±0.5℃, pH 6.6～6.9, aeration rate 0.8～1.1 vvm and agitation speed 300～400r/min.Under these conditions, the L-M168 strain could stably produce MTG higher than 2.75U/ml.

关 键 词：微生物谷氨酰胺转胺酶 分批发酵生产 链霉菌 培养基 在线监控技术 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]