米曲霉β-半乳糖苷酶的纯化与性质研究  

THE PURIFY AND PROPERTIES OF β-GALACTOSIDASE FROM ASPERGILLUS ORYZAE

作　　者：许牡丹[1] 范金波[1] 檀志芬[1] 何恩铭[1] 李宏杰[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西咸阳712081

出　　处：《陕西科技大学学报（自然科学版）》2003年第4期13-16,共4页Journal of Shaanxi University of Science & Technology

摘　　要：以40％饱和度的硫酸铵溶液去除杂蛋白,用80％饱和度的硫酸铵和75％的丙酮纯化米曲霉β—半乳糖苷醇,其比活力为147.7U/mg,回收率为38.1％。该酶最适反应温度和pH分别为50～55℃和4.0～6.0,作用于乳糖的米氏常数K_m为16.7mmol/L,最大反应速度V_(max)为5.56μmol/min。The β-galactosidase from aspergillus oryzae was purified by centrifuging, ammonium sulfate precipitation and acetone treating. The activity of purified β-galactosidase was 147. 7μ/mg. The recovery was 38. 1%. The optimum reactive temperature and pH for the enzyme were 50~55℃ and pH4. 0~ 6. 0,the Km for lactose was 16. 7μmol/L and Vmax was 5. 56umol/min

关 键 词：米曲霉 Β-半乳糖苷酶 纯化 性质 牛乳 乳清 低聚半乳糖 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]